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High expression of unsaturated fatty acid synthesis gene OLE1 in sake yeasts
GeneFilters® and Northern blot analysis revealed that the sake yeast strain Kyokai no. 7 (K7) showed a higher expression level of OLE1, which encodes a Δ-9 fatty acid desaturase gene, compared with the laboratory yeast strain X2180-1A. Other sake yeasts also showed a high expression level of OLE1. U...
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| Published in: | Journal of bioscience and bioengineering 2005-05, Vol.99 (5), p.512-516 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c478t-b1d5631a064a5a0420c2f0b7b499998ded6a33154db0b2adbea3f3523bcebad73 |
| container_end_page | 516 |
| container_issue | 5 |
| container_start_page | 512 |
| container_title | Journal of bioscience and bioengineering |
| container_volume | 99 |
| creator | Yamada, Tasuku Shimoi, Hitoshi Ito, Kiyoshi |
| description | GeneFilters® and Northern blot analysis revealed that the sake yeast strain Kyokai no. 7 (K7) showed a higher expression level of
OLE1, which encodes a Δ-9 fatty acid desaturase gene, compared with the laboratory yeast strain X2180-1A. Other sake yeasts also showed a high expression level of
OLE1. Unsaturated fatty acid concentrations in strain K7 are higher than that in strain X2180-1A, suggesting that the higher expression level of
OLE1 in sake yeasts increases the unsaturated fatty acid content in the cell membrane. Experiments using
OLE1 promoter:lacZ fusion reporter genes revealed that both the
cis element of the
OLE1 promoter and
trans factors are involved in the increased expression of
OLE1 in sake yeasts. |
| doi_str_mv | 10.1263/jbb.99.512 |
| format | article |
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OLE1, which encodes a Δ-9 fatty acid desaturase gene, compared with the laboratory yeast strain X2180-1A. Other sake yeasts also showed a high expression level of
OLE1. Unsaturated fatty acid concentrations in strain K7 are higher than that in strain X2180-1A, suggesting that the higher expression level of
OLE1 in sake yeasts increases the unsaturated fatty acid content in the cell membrane. Experiments using
OLE1 promoter:lacZ fusion reporter genes revealed that both the
cis element of the
OLE1 promoter and
trans factors are involved in the increased expression of
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OLE1, which encodes a Δ-9 fatty acid desaturase gene, compared with the laboratory yeast strain X2180-1A. Other sake yeasts also showed a high expression level of
OLE1. Unsaturated fatty acid concentrations in strain K7 are higher than that in strain X2180-1A, suggesting that the higher expression level of
OLE1 in sake yeasts increases the unsaturated fatty acid content in the cell membrane. Experiments using
OLE1 promoter:lacZ fusion reporter genes revealed that both the
cis element of the
OLE1 promoter and
trans factors are involved in the increased expression of
OLE1 in sake yeasts.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>BREWING</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENES</subject><subject>OLE1</subject><subject>PCR</subject><subject>SAKE YEAST</subject><subject>unsaturated fatty acid</subject><subject>UNSATURATED FATTY ACIDS</subject><issn>1389-1723</issn><issn>1347-4421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNptkM2LFDEQxRtRcB29eBcCogehZ_PZ6T7Ksh_KwO5Bz6GSVM9mnO0eU2lx_nuzzIIg1qUK6lfvFa9p3gq-FrJT5zvv18OwNkI-a86E0rbVWornj3M_tMJK9bJ5RbTjXFhuxVmzuUnbe4a_DxmJ0jyxeWTLRFCWDAUjG6GUI4OQIqPjVO6RErEtTshuN5eCpYkR_EB2RKBCr5sXI-wJ3zz1VfP96vLbxU27ub3-cvF50wZt-9J6EU2nBPBOgwGuJQ9y5N56PdTqI8YOlBJGR8-9hOgR1KiMVD6gh2jVqvl40j3k-eeCVNxDooD7PUw4L-SENUb1fV_B9_-Au3nJU_3NCa2FUtLUT1bNpxMV8kyUcXSHnB4gH53g7jFWV2N1w-BqrBX-8CQJFGA_ZphCor8XXbUduKjcuxM3wuxgmyvz9U5ybji3gzV1r097rEH9SpgdhYRTwJgyhuLinP5n_wcdCZG6</recordid><startdate>20050501</startdate><enddate>20050501</enddate><creator>Yamada, Tasuku</creator><creator>Shimoi, Hitoshi</creator><creator>Ito, Kiyoshi</creator><general>Elsevier B.V</general><general>Elsevier Science</general><general>Elsevier Limited</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope></search><sort><creationdate>20050501</creationdate><title>High expression of unsaturated fatty acid synthesis gene OLE1 in sake yeasts</title><author>Yamada, Tasuku ; Shimoi, Hitoshi ; Ito, Kiyoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-b1d5631a064a5a0420c2f0b7b499998ded6a33154db0b2adbea3f3523bcebad73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>BREWING</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENES</topic><topic>OLE1</topic><topic>PCR</topic><topic>SAKE YEAST</topic><topic>unsaturated fatty acid</topic><topic>UNSATURATED FATTY ACIDS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yamada, Tasuku</creatorcontrib><creatorcontrib>Shimoi, Hitoshi</creatorcontrib><creatorcontrib>Ito, Kiyoshi</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Journal of bioscience and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yamada, Tasuku</au><au>Shimoi, Hitoshi</au><au>Ito, Kiyoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High expression of unsaturated fatty acid synthesis gene OLE1 in sake yeasts</atitle><jtitle>Journal of bioscience and bioengineering</jtitle><date>2005-05-01</date><risdate>2005</risdate><volume>99</volume><issue>5</issue><spage>512</spage><epage>516</epage><pages>512-516</pages><issn>1389-1723</issn><eissn>1347-4421</eissn><coden>JFBIEX</coden><abstract>GeneFilters® and Northern blot analysis revealed that the sake yeast strain Kyokai no. 7 (K7) showed a higher expression level of
OLE1, which encodes a Δ-9 fatty acid desaturase gene, compared with the laboratory yeast strain X2180-1A. Other sake yeasts also showed a high expression level of
OLE1. Unsaturated fatty acid concentrations in strain K7 are higher than that in strain X2180-1A, suggesting that the higher expression level of
OLE1 in sake yeasts increases the unsaturated fatty acid content in the cell membrane. Experiments using
OLE1 promoter:lacZ fusion reporter genes revealed that both the
cis element of the
OLE1 promoter and
trans factors are involved in the increased expression of
OLE1 in sake yeasts.</abstract><cop>Amsterdarm</cop><pub>Elsevier B.V</pub><doi>10.1263/jbb.99.512</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1389-1723 |
| ispartof | Journal of bioscience and bioengineering, 2005-05, Vol.99 (5), p.512-516 |
| issn | 1389-1723 1347-4421 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17553888 |
| source | Elsevier |
| subjects | Biological and medical sciences Biotechnology BREWING Fundamental and applied biological sciences. Psychology GENES OLE1 PCR SAKE YEAST unsaturated fatty acid UNSATURATED FATTY ACIDS |
| title | High expression of unsaturated fatty acid synthesis gene OLE1 in sake yeasts |
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