Molecular phylogeny of the apterygotan insects based on nuclear and mitochondrial genes

• Published in: Pedobiologia 2000, Vol.44 (3), p.361-373
• Main Authors: Carapelli, Antonio, Frati, Francesco, Nardi, Francesco, Dallai, Romano, Simon, Chris
• Format: Article
• Language: English
• Subjects: 12S mitochondrial rRNA; Apterygota; Collembola; Diplura; Elongation Factor-1α; Microcoryphia; molecular phylogeny; Protura; secondary structure; Zygentoma
• ISSN: 0031-4056; 1873-1511
• DOI: 10.1078/S0031-4056(04)70055-4

Basal hexapodan orders (Apterygota) are traditionally divided in two well defined taxonomic groups, Entognatha and Ectognatha. Entognathy (the enclosed mouthparts condition) is considered the most distinctive character joining the Ellipura (Protura+ Collembola) with the order Diplura, whereas the presence of exposed mouthparts (ectognathy) is the feature shared by Microcoryphia and Zygentoma with the pterygote insects. In spite of the growing interest for the evolutionary history of the Apterygota, there is no complete agreement among the general phylogenetic hypotheses based on the study of morphological characters. In this study we analyzed the DNA sequence of segments of the nuclear Elongation Factor-1α(EF-1α) and of the mitochondrial 12S rRNA genes, and used different methods of phylogenetic reconstruction. The 12S gene seems to be more suited than the EF-1α to resolve some of the most outstanding systematic disputes, whereas the lack of resolution at the deeper nodes does not allow to assess the phylogenetic relationships within Microcoryphia and between ectognathan orders. We have obtained a fairly high support for the monophyly of the orders Diplura and Zygentoma. In the 12S analysis, the Ellipura and the Entognatha form monophyletic assemblages. In addition, the study of the distribution of introns in the EF-1α suggests a relationship between Collembola and Diplura.