In-vitro activities of paromomycin and lasalocid evaluated in combination against Cryptosporidium parvum

Using a chemiluminescence immunoassay, paromomycin and lasalocid were shown to inhibit Cryptosporidium parvum growth in Madin-Darby canine kidney cells in a concentration-dependent manner. The median effective concentrations (EC50s) for paromomycin and lasalocid were 1184 mg/L and 0.4 mg/L, respecti...

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Bibliographic Details
Published in:Journal of antimicrobial chemotherapy 1998-02, Vol.41 (2), p.293-296
Main Authors: XIANGDONG YOU, SCHINAZI, R. F, ARROWOOD, M. J, LEJKOWSKI, M, JUODAWLKIS, A. S, MEAD, J. R
Format: Article
Language:English
Subjects:
Animals
Antibacterial agents
Antibiotics. Antiinfectious agents. Antiparasitic agents
Biological and medical sciences
Cattle
Cryptosporidium parvum
Cryptosporidium parvum - drug effects
Cryptosporidium parvum - growth & development
Dogs
Dose-Response Relationship, Drug
Drug Therapy, Combination
Flow Cytometry
Immunoassay - methods
Kidney - cytology
Kidney - parasitology
lasalocid
Lasalocid - pharmacology
Lasalocid - toxicity
Luminescent Measurements
Madin-Darby canine kidney cells
Medical sciences
paromomycin
Paromomycin - pharmacology
Paromomycin - toxicity
Pharmacology. Drug treatments
Propidium - pharmacokinetics
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Summary:Using a chemiluminescence immunoassay, paromomycin and lasalocid were shown to inhibit Cryptosporidium parvum growth in Madin-Darby canine kidney cells in a concentration-dependent manner. The median effective concentrations (EC50s) for paromomycin and lasalocid were 1184 mg/L and 0.4 mg/L, respectively. Neither drug was cytotoxic to host cells at concentrations up to five times their EC50s. Drug combination studies were conducted and the resulting data were analysed by the median-effect principle and combination index method. Statistically significant synergy was observed when combinations of paromomycin and lasalocid were used at ratios of 5000:1 and 2500:1. A possible mechanism for synergy is discussed.
ISSN:0305-7453
1460-2091
1460-2091
DOI:10.1093/jac/41.2.293