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Localization of auxotrophic and benomyl resistance markers through the parasexual cycle in the Beauveria bassiana (Bals.) vuill entomopathogen
Genetic studies to localize auxotrophic markers and resistance to benomyl fungicide and to analyze gene transfer on the Beauveria bassiana deuteromycete were carried out using the parasexual cycle. Parasexual crosses among strains with complementary genetic markers resulted in vigorous heterocaryons...
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| Published in: | Journal of invertebrate pathology 1998-09, Vol.72 (2), p.119-125 |
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| Language: | English |
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| container_end_page | 125 |
| container_issue | 2 |
| container_start_page | 119 |
| container_title | Journal of invertebrate pathology |
| container_volume | 72 |
| creator | Bello, V.A. (Universidade Estadual de Londrina, Londrina, PR, Brazil.) Paccola-Meirelles, L.D |
| description | Genetic studies to localize auxotrophic markers and resistance to benomyl fungicide and to analyze gene transfer on the Beauveria bassiana deuteromycete were carried out using the parasexual cycle. Parasexual crosses among strains with complementary genetic markers resulted in vigorous heterocaryons. Selection of the segregant products was made using two methodologies: total isolation and parental elimination. Colonies with recombinant traits were recovered directly from the heterocaryon in all crosses, and no diploid colony was isolated. This shows the high instability of the diploid nucleus in this species. Among the parasexual segregant products preferential recovery of markers in one of the parental strains involved in the crosses was detected, probably because of an inhibition of conidiogenesis from one parental strain to another. Genetic markers were localized in four linkage groups by the parasexual crosses. In the first group markers nic4, nic3, thi2, bio3, ade2, ths2, and ben1R were localized; in the second, the marker met1 in the third, pab1; and in the fourth, bio1. The parental strain 196/A11/3 is a carrier of translocation among the linkage groups I and III |
| doi_str_mv | 10.1006/jipa.1998.4768 |
| format | article |
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(Universidade Estadual de Londrina, Londrina, PR, Brazil.) ; Paccola-Meirelles, L.D</creator><creatorcontrib>Bello, V.A. (Universidade Estadual de Londrina, Londrina, PR, Brazil.) ; Paccola-Meirelles, L.D</creatorcontrib><description>Genetic studies to localize auxotrophic markers and resistance to benomyl fungicide and to analyze gene transfer on the Beauveria bassiana deuteromycete were carried out using the parasexual cycle. Parasexual crosses among strains with complementary genetic markers resulted in vigorous heterocaryons. Selection of the segregant products was made using two methodologies: total isolation and parental elimination. Colonies with recombinant traits were recovered directly from the heterocaryon in all crosses, and no diploid colony was isolated. This shows the high instability of the diploid nucleus in this species. Among the parasexual segregant products preferential recovery of markers in one of the parental strains involved in the crosses was detected, probably because of an inhibition of conidiogenesis from one parental strain to another. Genetic markers were localized in four linkage groups by the parasexual crosses. In the first group markers nic4, nic3, thi2, bio3, ade2, ths2, and ben1R were localized; in the second, the marker met1 in the third, pab1; and in the fourth, bio1. The parental strain 196/A11/3 is a carrier of translocation among the linkage groups I and III</description><identifier>ISSN: 0022-2011</identifier><identifier>EISSN: 1096-0805</identifier><identifier>DOI: 10.1006/jipa.1998.4768</identifier><identifier>PMID: 9709011</identifier><identifier>CODEN: JIVPAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier</publisher><subject>AUXOTROPHIC MARKERS ; BEAUVERIA BASSIANA ; BENOMILO ; BENOMYL ; Biological and medical sciences ; CROSSBREDS ; ESTABILIDAD GENETICA ; FONGICIDE ; Fundamental and applied biological sciences. Psychology ; FUNGICIDAS ; FUNGICIDE TOLERANCE ; FUNGICIDES ; Fungicides, Industrial ; GENE LOCATION ; GENETIC ANALYSIS ; GENETIC MARKERS ; GENETIC STABILITY ; GENETICA ; GENETICS ; GENETIQUE ; Genic rearrangement. Recombination. Transposable element ; HETEROCARYON ; LINKAGE GROUPS ; LOCALISATION DE GENE ; LOCALIZACION DE GENES ; MARCADORES GENETICOS ; MARQUEUR GENETIQUE ; MITOSE ; MITOSIS ; Mitosporic Fungi - genetics ; MITOTIC INSTABILITY ; Molecular and cellular biology ; Molecular genetics ; PARASEXUALITY ; PRODUCTOS DEL CRUZAMIENTO ; PRODUIT DE CROISEMENT ; RECOMBINACION ; RECOMBINAISON ; RECOMBINATION ; Recombination, Genetic ; RESISTANCE AUX PRODUITS CHIMIQUES ; RESISTANCE TO CHEMICALS ; RESISTENCIA A PRODUCTOS QUIMICOS ; SEGREGACION ; SEGREGATION ; SEX ; SEXE ; SEXO ; STABILITE GENETIQUE ; STRAINS</subject><ispartof>Journal of invertebrate pathology, 1998-09, Vol.72 (2), p.119-125</ispartof><rights>1999 INIST-CNRS</rights><rights>Copyright 1998 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1621075$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9709011$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bello, V.A. (Universidade Estadual de Londrina, Londrina, PR, Brazil.)</creatorcontrib><creatorcontrib>Paccola-Meirelles, L.D</creatorcontrib><title>Localization of auxotrophic and benomyl resistance markers through the parasexual cycle in the Beauveria bassiana (Bals.) vuill entomopathogen</title><title>Journal of invertebrate pathology</title><addtitle>J Invertebr Pathol</addtitle><description>Genetic studies to localize auxotrophic markers and resistance to benomyl fungicide and to analyze gene transfer on the Beauveria bassiana deuteromycete were carried out using the parasexual cycle. Parasexual crosses among strains with complementary genetic markers resulted in vigorous heterocaryons. Selection of the segregant products was made using two methodologies: total isolation and parental elimination. Colonies with recombinant traits were recovered directly from the heterocaryon in all crosses, and no diploid colony was isolated. This shows the high instability of the diploid nucleus in this species. Among the parasexual segregant products preferential recovery of markers in one of the parental strains involved in the crosses was detected, probably because of an inhibition of conidiogenesis from one parental strain to another. Genetic markers were localized in four linkage groups by the parasexual crosses. In the first group markers nic4, nic3, thi2, bio3, ade2, ths2, and ben1R were localized; in the second, the marker met1 in the third, pab1; and in the fourth, bio1. The parental strain 196/A11/3 is a carrier of translocation among the linkage groups I and III</description><subject>AUXOTROPHIC MARKERS</subject><subject>BEAUVERIA BASSIANA</subject><subject>BENOMILO</subject><subject>BENOMYL</subject><subject>Biological and medical sciences</subject><subject>CROSSBREDS</subject><subject>ESTABILIDAD GENETICA</subject><subject>FONGICIDE</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>FUNGICIDAS</subject><subject>FUNGICIDE TOLERANCE</subject><subject>FUNGICIDES</subject><subject>Fungicides, Industrial</subject><subject>GENE LOCATION</subject><subject>GENETIC ANALYSIS</subject><subject>GENETIC MARKERS</subject><subject>GENETIC STABILITY</subject><subject>GENETICA</subject><subject>GENETICS</subject><subject>GENETIQUE</subject><subject>Genic rearrangement. Recombination. Transposable element</subject><subject>HETEROCARYON</subject><subject>LINKAGE GROUPS</subject><subject>LOCALISATION DE GENE</subject><subject>LOCALIZACION DE GENES</subject><subject>MARCADORES GENETICOS</subject><subject>MARQUEUR GENETIQUE</subject><subject>MITOSE</subject><subject>MITOSIS</subject><subject>Mitosporic Fungi - genetics</subject><subject>MITOTIC INSTABILITY</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>PARASEXUALITY</subject><subject>PRODUCTOS DEL CRUZAMIENTO</subject><subject>PRODUIT DE CROISEMENT</subject><subject>RECOMBINACION</subject><subject>RECOMBINAISON</subject><subject>RECOMBINATION</subject><subject>Recombination, Genetic</subject><subject>RESISTANCE AUX PRODUITS CHIMIQUES</subject><subject>RESISTANCE TO CHEMICALS</subject><subject>RESISTENCIA A PRODUCTOS QUIMICOS</subject><subject>SEGREGACION</subject><subject>SEGREGATION</subject><subject>SEX</subject><subject>SEXE</subject><subject>SEXO</subject><subject>STABILITE GENETIQUE</subject><subject>STRAINS</subject><issn>0022-2011</issn><issn>1096-0805</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNpFkU9v1DAQxS0EKkvhyg3JB4TgkGXsbBL7SCugSCv1UHqOZp3xrotjBzupunwIPjMWrOhp_rynJ81vGHstYC0A2o93bsK10FqtN12rnrCVAN1WoKB5ylYAUlYShHjOXuR8B6VrWn3GznQHugwr9nsbDXr3C2cXA4-W4_IQ5xSngzMcw8B3FOJ49DxRdnnGYIiPmH5Qynw-pLjsD6USnzBhpocFPTdH44m78Hd_QbjcU3LId5izw4D8_QX6vP7A7xfnPacwxzFOOB_insJL9swWlV6d6jm7_fL5--VVtb3--u3y07ayUsJcyWGwSrW6MRtR12gJ7dBJMhsFNTSNsYVFrTRK3UmrwehB600nmm6wqPWwq8_Zu3-5U4o_F8pzP7psyHsMFJfci65pS4Iqxjcn47Ibaein5Mr5x_4EsOhvTzrmAtKmQsjl_zbRSgFd8xhjMfa4T8Vye1O-pgFqBar-A83oivs</recordid><startdate>19980901</startdate><enddate>19980901</enddate><creator>Bello, V.A. (Universidade Estadual de Londrina, Londrina, PR, Brazil.)</creator><creator>Paccola-Meirelles, L.D</creator><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>M7N</scope></search><sort><creationdate>19980901</creationdate><title>Localization of auxotrophic and benomyl resistance markers through the parasexual cycle in the Beauveria bassiana (Bals.) vuill entomopathogen</title><author>Bello, V.A. (Universidade Estadual de Londrina, Londrina, PR, Brazil.) ; Paccola-Meirelles, L.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f220t-2ddf88695c4133afeafd72ec4803055cf476389a2972f90c9d9947157dfa99db3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>AUXOTROPHIC MARKERS</topic><topic>BEAUVERIA BASSIANA</topic><topic>BENOMILO</topic><topic>BENOMYL</topic><topic>Biological and medical sciences</topic><topic>CROSSBREDS</topic><topic>ESTABILIDAD GENETICA</topic><topic>FONGICIDE</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>FUNGICIDAS</topic><topic>FUNGICIDE TOLERANCE</topic><topic>FUNGICIDES</topic><topic>Fungicides, Industrial</topic><topic>GENE LOCATION</topic><topic>GENETIC ANALYSIS</topic><topic>GENETIC MARKERS</topic><topic>GENETIC STABILITY</topic><topic>GENETICA</topic><topic>GENETICS</topic><topic>GENETIQUE</topic><topic>Genic rearrangement. Recombination. Transposable element</topic><topic>HETEROCARYON</topic><topic>LINKAGE GROUPS</topic><topic>LOCALISATION DE GENE</topic><topic>LOCALIZACION DE GENES</topic><topic>MARCADORES GENETICOS</topic><topic>MARQUEUR GENETIQUE</topic><topic>MITOSE</topic><topic>MITOSIS</topic><topic>Mitosporic Fungi - genetics</topic><topic>MITOTIC INSTABILITY</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>PARASEXUALITY</topic><topic>PRODUCTOS DEL CRUZAMIENTO</topic><topic>PRODUIT DE CROISEMENT</topic><topic>RECOMBINACION</topic><topic>RECOMBINAISON</topic><topic>RECOMBINATION</topic><topic>Recombination, Genetic</topic><topic>RESISTANCE AUX PRODUITS CHIMIQUES</topic><topic>RESISTANCE TO CHEMICALS</topic><topic>RESISTENCIA A PRODUCTOS QUIMICOS</topic><topic>SEGREGACION</topic><topic>SEGREGATION</topic><topic>SEX</topic><topic>SEXE</topic><topic>SEXO</topic><topic>STABILITE GENETIQUE</topic><topic>STRAINS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bello, V.A. (Universidade Estadual de Londrina, Londrina, PR, Brazil.)</creatorcontrib><creatorcontrib>Paccola-Meirelles, L.D</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Journal of invertebrate pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bello, V.A. (Universidade Estadual de Londrina, Londrina, PR, Brazil.)</au><au>Paccola-Meirelles, L.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Localization of auxotrophic and benomyl resistance markers through the parasexual cycle in the Beauveria bassiana (Bals.) vuill entomopathogen</atitle><jtitle>Journal of invertebrate pathology</jtitle><addtitle>J Invertebr Pathol</addtitle><date>1998-09-01</date><risdate>1998</risdate><volume>72</volume><issue>2</issue><spage>119</spage><epage>125</epage><pages>119-125</pages><issn>0022-2011</issn><eissn>1096-0805</eissn><coden>JIVPAZ</coden><abstract>Genetic studies to localize auxotrophic markers and resistance to benomyl fungicide and to analyze gene transfer on the Beauveria bassiana deuteromycete were carried out using the parasexual cycle. Parasexual crosses among strains with complementary genetic markers resulted in vigorous heterocaryons. Selection of the segregant products was made using two methodologies: total isolation and parental elimination. Colonies with recombinant traits were recovered directly from the heterocaryon in all crosses, and no diploid colony was isolated. This shows the high instability of the diploid nucleus in this species. Among the parasexual segregant products preferential recovery of markers in one of the parental strains involved in the crosses was detected, probably because of an inhibition of conidiogenesis from one parental strain to another. Genetic markers were localized in four linkage groups by the parasexual crosses. In the first group markers nic4, nic3, thi2, bio3, ade2, ths2, and ben1R were localized; in the second, the marker met1 in the third, pab1; and in the fourth, bio1. The parental strain 196/A11/3 is a carrier of translocation among the linkage groups I and III</abstract><cop>Amsterdam</cop><pub>Elsevier</pub><pmid>9709011</pmid><doi>10.1006/jipa.1998.4768</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of invertebrate pathology, 1998-09, Vol.72 (2), p.119-125 |
| issn | 0022-2011 1096-0805 |
| language | eng |
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| source | Elsevier:Jisc Collections:Elsevier Read and Publish Agreement 2022-2024:Freedom Collection (Reading list) |
| subjects | AUXOTROPHIC MARKERS BEAUVERIA BASSIANA BENOMILO BENOMYL Biological and medical sciences CROSSBREDS ESTABILIDAD GENETICA FONGICIDE Fundamental and applied biological sciences. Psychology FUNGICIDAS FUNGICIDE TOLERANCE FUNGICIDES Fungicides, Industrial GENE LOCATION GENETIC ANALYSIS GENETIC MARKERS GENETIC STABILITY GENETICA GENETICS GENETIQUE Genic rearrangement. Recombination. Transposable element HETEROCARYON LINKAGE GROUPS LOCALISATION DE GENE LOCALIZACION DE GENES MARCADORES GENETICOS MARQUEUR GENETIQUE MITOSE MITOSIS Mitosporic Fungi - genetics MITOTIC INSTABILITY Molecular and cellular biology Molecular genetics PARASEXUALITY PRODUCTOS DEL CRUZAMIENTO PRODUIT DE CROISEMENT RECOMBINACION RECOMBINAISON RECOMBINATION Recombination, Genetic RESISTANCE AUX PRODUITS CHIMIQUES RESISTANCE TO CHEMICALS RESISTENCIA A PRODUCTOS QUIMICOS SEGREGACION SEGREGATION SEX SEXE SEXO STABILITE GENETIQUE STRAINS |
| title | Localization of auxotrophic and benomyl resistance markers through the parasexual cycle in the Beauveria bassiana (Bals.) vuill entomopathogen |
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