Identification of Colletotrichum species responsible for anthracnose of strawberry based on the internal transcribed spacers of the ribosomal region

In recent years, different molecular techniques have led to an important progress in the characterisation of Colletotrichum species, but there are no available methods which permit the easy identification of Colletotrichum strains and their assignation to classical species. In the present work, the...

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Bibliographic Details
Published in:FEMS microbiology letters 2000-08, Vol.189 (1), p.97-101
Main Authors: Martı́nez-Culebras, Pedro V., Barrio, Eladio, Garcı́a, M.Dolores, Querol, Amparo
Format: Article
Language:English
Subjects:
Colletotrichum
Colletotrichum fragariae
Colletotrichum gloeosporioides
Colletotrichum species
endonuclease
endonucleases
Fragaria ananassa
Identification
internal transcribed spacers
rDNA restriction analysis
rRNA 5.8S
Strawberry anthracnose
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Summary:In recent years, different molecular techniques have led to an important progress in the characterisation of Colletotrichum species, but there are no available methods which permit the easy identification of Colletotrichum strains and their assignation to classical species. In the present work, the restriction patterns generated from the region spanning the internal transcribed spacers (ITS1 and ITS2) and the 5.8S rRNA gene, were used to identify a total of 80 strains of Colletotrichum, the majority of them isolated from strawberry. One of the most interesting results derived from this study was the easy and reliable distinction, using the endonuclease MvnI, between Colletotrichum fragariae and Colletotrichum gloeosporoides, both responsible of anthracnose on strawberry and phenotypically indistinguishable. Moreover, we propose the restriction fragments generated by the endonucleases MvnI, PvuII and ScrFI as a rapid method to differentiate species of the Colletotrichum genus.
ISSN:0378-1097
1574-6968
DOI:10.1016/S0378-1097(00)00260-3