Loading…
Molecular characterization of susceptible and naturally resistant strains of Trypanosoma cruzi to benznidazole and nifurtimox
Twenty-seven Trypanosoma cruzi strains, susceptible or naturally resistant to the nitroderivatives benznidazole and nifurtimox, were analyzed using the following molecular markers: (i) isoenzyme patterns of six enzymes; (ii) genetic variability assayed by randomly amplified polymorphic DNA (RAPD) wi...
Saved in:
| Published in: | Molecular and biochemical parasitology 1998-06, Vol.93 (2), p.203-214 |
|---|---|
| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c511t-acf595ad8af6fae8bab8555f9b2f5df72f14c7e6400e0aecd7d3b960f06f49e03 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c511t-acf595ad8af6fae8bab8555f9b2f5df72f14c7e6400e0aecd7d3b960f06f49e03 |
| container_end_page | 214 |
| container_issue | 2 |
| container_start_page | 203 |
| container_title | Molecular and biochemical parasitology |
| container_volume | 93 |
| creator | Murta, Silvane M.F Gazzinelli, Ricardo T Brener, Zigman Romanha, Alvaro J |
| description | Twenty-seven
Trypanosoma cruzi strains, susceptible or naturally resistant to the nitroderivatives benznidazole and nifurtimox, were analyzed using the following molecular markers: (i) isoenzyme patterns of six enzymes; (ii) genetic variability assayed by randomly amplified polymorphic DNA (RAPD) with two different primers; and (iii) gene probes for P-glycoprotein (TcPGP), hypoxanthine-guanine phosphoribosyltransferase (HGPRT), the ribosomal RNA gene (
rDNA) and the mini-exon gene (
MEX). RAPD and isoenzyme profiles divided the
T. cruzi strains into three groups, whereas the gene probes divided the
T. cruzi strains in two groups. Strains classified as group I or II by RAPD or zymodemes Z1 or Z2 by isoenzyme analysis were either susceptible or naturally resistant to the nitroderivatives. In contrast, strains classified as group III by RAPD and zymodeme ZB by isoenzyme analysis were only drug susceptible and showed polymorphisms for
HGPRT and
TcPGP. No correlation was observed between drug susceptibility and polymorphisms of
rDNA and
MEX. Eighteen
T. cruzi strains isolated from different geographic regions were included in this study. Thus, from a total of 45
T. cruzi strains analyzed, all 19 of zymodeme B were susceptible to the experimental treatment independent of their geographic origin. |
| doi_str_mv | 10.1016/S0166-6851(98)00037-1 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_17572697</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0166685198000371</els_id><sourcerecordid>17572697</sourcerecordid><originalsourceid>FETCH-LOGICAL-c511t-acf595ad8af6fae8bab8555f9b2f5df72f14c7e6400e0aecd7d3b960f06f49e03</originalsourceid><addsrcrecordid>eNqFkE1PGzEQhq2qCFLoT0DyqSqHbe1NbK9PVYXohwTiAJytWXusutq1g-2tmkj972xIypXLzGGemdH7EHLO2SfOuPx8NxfZyE7wj7q7YIwtVcPfkAXvVNvoVdu9JYsX5IS8K-X3DAkl5TE51lK2iokF-XeTBrTTAJnaX5DBVsxhCzWkSJOnZSoW1zX0A1KIjkaoU4Zh2NCMJZQKsdJSM4RYdvh93qwhppJGoDZP20Broj3GbQwOtun_keCnXMOY_p6RIw9DwfeHfkoevl3dX_5orm-__7z8et1YwXltwHqhBbgOvPSAXQ99J4Twum-9cF61nq-sQrliDBmgdcotey2ZZ9KvNLLlKfmwv7vO6XHCUs0Y5mDDABHTVAxXQrVSqxkUe9DmVEpGb9Y5jJA3hjOz026etZudU6M786zd8Hnv_PBg6kd0L1sHz_P8y36Oc8o_AbMpNmC06EJGW41L4ZUPT9VWlzM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17572697</pqid></control><display><type>article</type><title>Molecular characterization of susceptible and naturally resistant strains of Trypanosoma cruzi to benznidazole and nifurtimox</title><source>ScienceDirect Journals</source><creator>Murta, Silvane M.F ; Gazzinelli, Ricardo T ; Brener, Zigman ; Romanha, Alvaro J</creator><creatorcontrib>Murta, Silvane M.F ; Gazzinelli, Ricardo T ; Brener, Zigman ; Romanha, Alvaro J</creatorcontrib><description>Twenty-seven
Trypanosoma cruzi strains, susceptible or naturally resistant to the nitroderivatives benznidazole and nifurtimox, were analyzed using the following molecular markers: (i) isoenzyme patterns of six enzymes; (ii) genetic variability assayed by randomly amplified polymorphic DNA (RAPD) with two different primers; and (iii) gene probes for P-glycoprotein (TcPGP), hypoxanthine-guanine phosphoribosyltransferase (HGPRT), the ribosomal RNA gene (
rDNA) and the mini-exon gene (
MEX). RAPD and isoenzyme profiles divided the
T. cruzi strains into three groups, whereas the gene probes divided the
T. cruzi strains in two groups. Strains classified as group I or II by RAPD or zymodemes Z1 or Z2 by isoenzyme analysis were either susceptible or naturally resistant to the nitroderivatives. In contrast, strains classified as group III by RAPD and zymodeme ZB by isoenzyme analysis were only drug susceptible and showed polymorphisms for
HGPRT and
TcPGP. No correlation was observed between drug susceptibility and polymorphisms of
rDNA and
MEX. Eighteen
T. cruzi strains isolated from different geographic regions were included in this study. Thus, from a total of 45
T. cruzi strains analyzed, all 19 of zymodeme B were susceptible to the experimental treatment independent of their geographic origin.</description><identifier>ISSN: 0166-6851</identifier><identifier>EISSN: 1872-9428</identifier><identifier>DOI: 10.1016/S0166-6851(98)00037-1</identifier><identifier>PMID: 9662705</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; ATP Binding Cassette Transporter, Subfamily B, Member 1 - genetics ; benznidazole ; Brazil ; Chagas Disease - parasitology ; Chemotherapy ; DNA, Protozoan - genetics ; DNA, Ribosomal - genetics ; Drug Resistance ; Exons - genetics ; Genes, Protozoan ; Genetic Variation ; Glucose-6-Phosphate Isomerase - analysis ; Humans ; Hypoxanthine Phosphoribosyltransferase - genetics ; hypoxanthine-guanine phosphoribosyltransferase ; Isoenzymes - analysis ; MEX protein ; nifurtimox ; Nifurtimox - pharmacology ; Nitroimidazoles - pharmacology ; Polymorphism, Genetic ; Random Amplified Polymorphic DNA Technique ; Randomly amplified polymorphic DNA ; Trypanocidal Agents - pharmacology ; Trypanosoma cruzi ; Trypanosoma cruzi - classification ; Trypanosoma cruzi - drug effects ; Trypanosoma cruzi - enzymology ; Trypanosoma cruzi - genetics ; Zymodeme ; zymodeme B</subject><ispartof>Molecular and biochemical parasitology, 1998-06, Vol.93 (2), p.203-214</ispartof><rights>1998 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c511t-acf595ad8af6fae8bab8555f9b2f5df72f14c7e6400e0aecd7d3b960f06f49e03</citedby><cites>FETCH-LOGICAL-c511t-acf595ad8af6fae8bab8555f9b2f5df72f14c7e6400e0aecd7d3b960f06f49e03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9662705$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Murta, Silvane M.F</creatorcontrib><creatorcontrib>Gazzinelli, Ricardo T</creatorcontrib><creatorcontrib>Brener, Zigman</creatorcontrib><creatorcontrib>Romanha, Alvaro J</creatorcontrib><title>Molecular characterization of susceptible and naturally resistant strains of Trypanosoma cruzi to benznidazole and nifurtimox</title><title>Molecular and biochemical parasitology</title><addtitle>Mol Biochem Parasitol</addtitle><description>Twenty-seven
Trypanosoma cruzi strains, susceptible or naturally resistant to the nitroderivatives benznidazole and nifurtimox, were analyzed using the following molecular markers: (i) isoenzyme patterns of six enzymes; (ii) genetic variability assayed by randomly amplified polymorphic DNA (RAPD) with two different primers; and (iii) gene probes for P-glycoprotein (TcPGP), hypoxanthine-guanine phosphoribosyltransferase (HGPRT), the ribosomal RNA gene (
rDNA) and the mini-exon gene (
MEX). RAPD and isoenzyme profiles divided the
T. cruzi strains into three groups, whereas the gene probes divided the
T. cruzi strains in two groups. Strains classified as group I or II by RAPD or zymodemes Z1 or Z2 by isoenzyme analysis were either susceptible or naturally resistant to the nitroderivatives. In contrast, strains classified as group III by RAPD and zymodeme ZB by isoenzyme analysis were only drug susceptible and showed polymorphisms for
HGPRT and
TcPGP. No correlation was observed between drug susceptibility and polymorphisms of
rDNA and
MEX. Eighteen
T. cruzi strains isolated from different geographic regions were included in this study. Thus, from a total of 45
T. cruzi strains analyzed, all 19 of zymodeme B were susceptible to the experimental treatment independent of their geographic origin.</description><subject>Animals</subject><subject>ATP Binding Cassette Transporter, Subfamily B, Member 1 - genetics</subject><subject>benznidazole</subject><subject>Brazil</subject><subject>Chagas Disease - parasitology</subject><subject>Chemotherapy</subject><subject>DNA, Protozoan - genetics</subject><subject>DNA, Ribosomal - genetics</subject><subject>Drug Resistance</subject><subject>Exons - genetics</subject><subject>Genes, Protozoan</subject><subject>Genetic Variation</subject><subject>Glucose-6-Phosphate Isomerase - analysis</subject><subject>Humans</subject><subject>Hypoxanthine Phosphoribosyltransferase - genetics</subject><subject>hypoxanthine-guanine phosphoribosyltransferase</subject><subject>Isoenzymes - analysis</subject><subject>MEX protein</subject><subject>nifurtimox</subject><subject>Nifurtimox - pharmacology</subject><subject>Nitroimidazoles - pharmacology</subject><subject>Polymorphism, Genetic</subject><subject>Random Amplified Polymorphic DNA Technique</subject><subject>Randomly amplified polymorphic DNA</subject><subject>Trypanocidal Agents - pharmacology</subject><subject>Trypanosoma cruzi</subject><subject>Trypanosoma cruzi - classification</subject><subject>Trypanosoma cruzi - drug effects</subject><subject>Trypanosoma cruzi - enzymology</subject><subject>Trypanosoma cruzi - genetics</subject><subject>Zymodeme</subject><subject>zymodeme B</subject><issn>0166-6851</issn><issn>1872-9428</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNqFkE1PGzEQhq2qCFLoT0DyqSqHbe1NbK9PVYXohwTiAJytWXusutq1g-2tmkj972xIypXLzGGemdH7EHLO2SfOuPx8NxfZyE7wj7q7YIwtVcPfkAXvVNvoVdu9JYsX5IS8K-X3DAkl5TE51lK2iokF-XeTBrTTAJnaX5DBVsxhCzWkSJOnZSoW1zX0A1KIjkaoU4Zh2NCMJZQKsdJSM4RYdvh93qwhppJGoDZP20Broj3GbQwOtun_keCnXMOY_p6RIw9DwfeHfkoevl3dX_5orm-__7z8et1YwXltwHqhBbgOvPSAXQ99J4Twum-9cF61nq-sQrliDBmgdcotey2ZZ9KvNLLlKfmwv7vO6XHCUs0Y5mDDABHTVAxXQrVSqxkUe9DmVEpGb9Y5jJA3hjOz026etZudU6M786zd8Hnv_PBg6kd0L1sHz_P8y36Oc8o_AbMpNmC06EJGW41L4ZUPT9VWlzM</recordid><startdate>19980601</startdate><enddate>19980601</enddate><creator>Murta, Silvane M.F</creator><creator>Gazzinelli, Ricardo T</creator><creator>Brener, Zigman</creator><creator>Romanha, Alvaro J</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope></search><sort><creationdate>19980601</creationdate><title>Molecular characterization of susceptible and naturally resistant strains of Trypanosoma cruzi to benznidazole and nifurtimox</title><author>Murta, Silvane M.F ; Gazzinelli, Ricardo T ; Brener, Zigman ; Romanha, Alvaro J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c511t-acf595ad8af6fae8bab8555f9b2f5df72f14c7e6400e0aecd7d3b960f06f49e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>ATP Binding Cassette Transporter, Subfamily B, Member 1 - genetics</topic><topic>benznidazole</topic><topic>Brazil</topic><topic>Chagas Disease - parasitology</topic><topic>Chemotherapy</topic><topic>DNA, Protozoan - genetics</topic><topic>DNA, Ribosomal - genetics</topic><topic>Drug Resistance</topic><topic>Exons - genetics</topic><topic>Genes, Protozoan</topic><topic>Genetic Variation</topic><topic>Glucose-6-Phosphate Isomerase - analysis</topic><topic>Humans</topic><topic>Hypoxanthine Phosphoribosyltransferase - genetics</topic><topic>hypoxanthine-guanine phosphoribosyltransferase</topic><topic>Isoenzymes - analysis</topic><topic>MEX protein</topic><topic>nifurtimox</topic><topic>Nifurtimox - pharmacology</topic><topic>Nitroimidazoles - pharmacology</topic><topic>Polymorphism, Genetic</topic><topic>Random Amplified Polymorphic DNA Technique</topic><topic>Randomly amplified polymorphic DNA</topic><topic>Trypanocidal Agents - pharmacology</topic><topic>Trypanosoma cruzi</topic><topic>Trypanosoma cruzi - classification</topic><topic>Trypanosoma cruzi - drug effects</topic><topic>Trypanosoma cruzi - enzymology</topic><topic>Trypanosoma cruzi - genetics</topic><topic>Zymodeme</topic><topic>zymodeme B</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Murta, Silvane M.F</creatorcontrib><creatorcontrib>Gazzinelli, Ricardo T</creatorcontrib><creatorcontrib>Brener, Zigman</creatorcontrib><creatorcontrib>Romanha, Alvaro J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Molecular and biochemical parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Murta, Silvane M.F</au><au>Gazzinelli, Ricardo T</au><au>Brener, Zigman</au><au>Romanha, Alvaro J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization of susceptible and naturally resistant strains of Trypanosoma cruzi to benznidazole and nifurtimox</atitle><jtitle>Molecular and biochemical parasitology</jtitle><addtitle>Mol Biochem Parasitol</addtitle><date>1998-06-01</date><risdate>1998</risdate><volume>93</volume><issue>2</issue><spage>203</spage><epage>214</epage><pages>203-214</pages><issn>0166-6851</issn><eissn>1872-9428</eissn><abstract>Twenty-seven
Trypanosoma cruzi strains, susceptible or naturally resistant to the nitroderivatives benznidazole and nifurtimox, were analyzed using the following molecular markers: (i) isoenzyme patterns of six enzymes; (ii) genetic variability assayed by randomly amplified polymorphic DNA (RAPD) with two different primers; and (iii) gene probes for P-glycoprotein (TcPGP), hypoxanthine-guanine phosphoribosyltransferase (HGPRT), the ribosomal RNA gene (
rDNA) and the mini-exon gene (
MEX). RAPD and isoenzyme profiles divided the
T. cruzi strains into three groups, whereas the gene probes divided the
T. cruzi strains in two groups. Strains classified as group I or II by RAPD or zymodemes Z1 or Z2 by isoenzyme analysis were either susceptible or naturally resistant to the nitroderivatives. In contrast, strains classified as group III by RAPD and zymodeme ZB by isoenzyme analysis were only drug susceptible and showed polymorphisms for
HGPRT and
TcPGP. No correlation was observed between drug susceptibility and polymorphisms of
rDNA and
MEX. Eighteen
T. cruzi strains isolated from different geographic regions were included in this study. Thus, from a total of 45
T. cruzi strains analyzed, all 19 of zymodeme B were susceptible to the experimental treatment independent of their geographic origin.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>9662705</pmid><doi>10.1016/S0166-6851(98)00037-1</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0166-6851 |
| ispartof | Molecular and biochemical parasitology, 1998-06, Vol.93 (2), p.203-214 |
| issn | 0166-6851 1872-9428 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17572697 |
| source | ScienceDirect Journals |
| subjects | Animals ATP Binding Cassette Transporter, Subfamily B, Member 1 - genetics benznidazole Brazil Chagas Disease - parasitology Chemotherapy DNA, Protozoan - genetics DNA, Ribosomal - genetics Drug Resistance Exons - genetics Genes, Protozoan Genetic Variation Glucose-6-Phosphate Isomerase - analysis Humans Hypoxanthine Phosphoribosyltransferase - genetics hypoxanthine-guanine phosphoribosyltransferase Isoenzymes - analysis MEX protein nifurtimox Nifurtimox - pharmacology Nitroimidazoles - pharmacology Polymorphism, Genetic Random Amplified Polymorphic DNA Technique Randomly amplified polymorphic DNA Trypanocidal Agents - pharmacology Trypanosoma cruzi Trypanosoma cruzi - classification Trypanosoma cruzi - drug effects Trypanosoma cruzi - enzymology Trypanosoma cruzi - genetics Zymodeme zymodeme B |
| title | Molecular characterization of susceptible and naturally resistant strains of Trypanosoma cruzi to benznidazole and nifurtimox |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T03%3A55%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20characterization%20of%20susceptible%20and%20naturally%20resistant%20strains%20of%20Trypanosoma%20cruzi%20to%20benznidazole%20and%20nifurtimox&rft.jtitle=Molecular%20and%20biochemical%20parasitology&rft.au=Murta,%20Silvane%20M.F&rft.date=1998-06-01&rft.volume=93&rft.issue=2&rft.spage=203&rft.epage=214&rft.pages=203-214&rft.issn=0166-6851&rft.eissn=1872-9428&rft_id=info:doi/10.1016/S0166-6851(98)00037-1&rft_dat=%3Cproquest_cross%3E17572697%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c511t-acf595ad8af6fae8bab8555f9b2f5df72f14c7e6400e0aecd7d3b960f06f49e03%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=17572697&rft_id=info:pmid/9662705&rfr_iscdi=true |