Expression of Aspergillus aculeatus No. F-50 cellobiohydrolase I (cbhI) and beta-glucosidase 1 (bgl1) genes by Saccharomyces cerevisiae

A cellobiohydrolase I (cbhI) and a beta-glucosidase 1 (bgl1) gene of Aspergillus aculeatus were expressed in Saccharomyces cerevisiae. The transformed cells secreted the enzymes efficiently in an active form. The recombinant CBHI gave two bands of different molecular mass (110 and 90 kDa) and the re...

Full description

Saved in:
Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1998-08, Vol.62 (8), p.1615-1618
Main Authors: Takada, G. (Osaka Prefectural Univ., Sakai (Japan). Coll. of Agriculture), Kawaguchi, T, Sumitani, J, Arai, M
Format: Article
Language:English
Subjects:
ASPERGILLUS
Aspergillus - enzymology
Aspergillus - genetics
Aspergillus aculeatus
BETA GLUCOSIDASA
BETA GLUCOSIDASE
beta-Glucosidase - biosynthesis
beta-Glucosidase - chemistry
beta-Glucosidase - genetics
bgl1 gene
Biological and medical sciences
Biotechnology
cbhI gene
cellobiohydrolase
cellobiohydrolase I
CELLOBIOSE
Cellulase - biosynthesis
Cellulase - chemistry
Cellulase - genetics
Cellulose - chemistry
Cellulose 1,4-beta-Cellobiosidase
CELOBIOSA
Electrophoresis, Polyacrylamide Gel
EXPRESION GENICA
EXPRESSION DES GENES
Fundamental and applied biological sciences. Psychology
GENE EXPRESSION
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Fungal
Genetic engineering
Genetic technics
Genetic Vectors
HIDROLASAS
HYDROLASE
HYDROLASES
Methods. Procedures. Technologies
Modification of gene expression level
Molecular Weight
Recombinant Proteins - biosynthesis
Recombinant Proteins - chemistry
Recombinant Proteins - genetics
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Substrate Specificity
synergism
β-glucosidase
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A cellobiohydrolase I (cbhI) and a beta-glucosidase 1 (bgl1) gene of Aspergillus aculeatus were expressed in Saccharomyces cerevisiae. The transformed cells secreted the enzymes efficiently in an active form. The recombinant CBHI gave two bands of different molecular mass (110 and 90 kDa) and the recombinant BGL1 gave one band (180 kDa) by SDS-PAGE. The recombinant CBHI and BGL1 had the same enzymatical properties as the native enzyme except for the specific activity toward cellulosic substrates. By the combination of three different types of cellulases, FI-CMCase, CBHI, and BGL1, we could hydrolyze Avicel up to 59% under our experimental conditions
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.62.1615