Detoxification of Corn Stover and Corn Starch Pyrolysis Liquors by Ligninolytic Enzymes of Phanerochaete chrysosporium

Phanerochaete chrysosporium (ATCC 24725) shake flask culture with 3 mM veratryl alcohol addition on day 3 was able to grow and detoxify different concentrations of diluted corn stover (Dcs) and diluted corn starch (Dst) pyrolysis liquors [10, 25, and 50% (v/v)] in defined media. GC-MS analysis of re...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2005-04, Vol.53 (8), p.2969-2977
Main Authors: Khiyami, Mohammad A, Pometto, Anthony L, Brown, Robert C
Format: Article
Language:English
Subjects:
Biological and medical sciences
biomass
Cereal and baking product industries
corn starch
corn stover
Cycloheximide - pharmacology
enzyme activity
Food industries
Fundamental and applied biological sciences. Psychology
Gas Chromatography-Mass Spectrometry
Hot Temperature
Inactivation, Metabolic
lactic acid
Lactic Acid - biosynthesis
lactic fermentation
Lactobacillus casei
Lactobacillus casei - drug effects
Lactobacillus casei - growth & development
Lactobacillus casei - metabolism
Lignin - metabolism
lignin peroxidase
metabolic detoxification
Phanerochaete - enzymology
Phanerochaete chrysosporium
phenolic compounds
Phenols - metabolism
pyrolysis
pyrolysis liquors
Sodium Azide - pharmacology
Starch - chemistry
Starch - metabolism
Starch and starchy product industries
Zea mays - chemistry
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Summary:Phanerochaete chrysosporium (ATCC 24725) shake flask culture with 3 mM veratryl alcohol addition on day 3 was able to grow and detoxify different concentrations of diluted corn stover (Dcs) and diluted corn starch (Dst) pyrolysis liquors [10, 25, and 50% (v/v)] in defined media. GC-MS analysis of reaction products showed a decrease and change in some compounds. In addition, the total phenolic assay with Dcs samples demonstrated a decrease in the phenolic compounds. A bioassay employing Lactobacillus casei growth and lactic acid production was developed to confirm the removal of toxic compounds from 10 and 25% (v/v) Dcs and Dst by the lignolytic enzymes, but not from 50% (v/v) Dcs and Dst. The removal did not occur when sodium azide or cycloheximide was added to Ph. chrysosporium culture media, confirming the participation of lignolytic enzymes in the detoxification process. A concentrated enzyme preparation decreased the phenolic compounds in 10% (v/v) corn stover and corn starch pyrolysis liquors to the same extent as the fungal cultures. Keywords: Corn stover; corn starch; detoxification; lignolytic enzymes
ISSN:0021-8561
1520-5118
DOI:10.1021/jf048223m