Detection of Clostridium novyi type B α toxin by cell culture systems

Ten permanent cell lines were examined for their reaction to the Clostridium novyi α toxin. The action of the toxin was determined after 3 days by microscopic examination and the MTT assay. The α toxin exhibited the strongest effect on ESH-L cells rather than other cell lines. Vero and SFT-R cells r...

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Bibliographic Details
Published in:FEMS immunology and medical microbiology 1999-07, Vol.24 (3), p.275-280
Main Authors: Borrmann, Erika, Schulze, Frank
Format: Article
Language:English
Subjects:
a-Toxin
Alpha toxin
Animals
Bacterial Toxins - toxicity
Cattle
Cell culture
Cell Culture Techniques - methods
Cell Line
Cercopithecus aethiops
CHO Cells
Clostridium
Clostridium novyi
Cricetinae
Detection
Dogs
Macaca mulatta
Mice
pathogens
Rats
Sheep
Vero Cells
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Summary:Ten permanent cell lines were examined for their reaction to the Clostridium novyi α toxin. The action of the toxin was determined after 3 days by microscopic examination and the MTT assay. The α toxin exhibited the strongest effect on ESH-L cells rather than other cell lines. Vero and SFT-R cells reacted in a comparable way, but less sensitively. We were able to show that the cytopathic effect on the three types of cells was neutralised by the international standard for gas gangrene antitoxin ( C. novyi) but in no case by heterologous antisera. Our results have shown that the three cell lines were specific indicators for the detection of the cytopathic effect of α toxin. The cytopathic effect can be measured reproducibly by the cell culture assay used. These results are suitable as the starting point for the development of the neutralisation test using cell cultures.
ISSN:0928-8244
1574-695X
DOI:10.1016/S0928-8244(99)00042-5