Effect of acupuncture on 6-hydroxydopamine-induced nigrostratal dopaminergic neuronal cell death in rats

• Published in: Neuroscience letters 2005-08, Vol.384 (1), p.133-138
• Main Authors: Kim, Yeung-Kee, Lim, Hyung-Ho, Song, Yun-Kyung, Lee, Hee-Hyuk, Lim, Sabina, Han, Seung-Moo, Kim, Chang-Ju
• Format: Article
• Language: English
• Subjects: 6-Hydroxydopamine; Acupuncture; Acupuncture - methods; Adrenergic Agents; Animals; Apomorphine - pharmacology; Behavior, Animal - drug effects; Biological and medical sciences; Cell Count - methods; Cell Death - drug effects; Corpus Striatum - drug effects; Disease Models, Animal; Dopamine - metabolism; Fundamental and applied biological sciences. Psychology; Immunohistochemistry - methods; Male; Medical sciences; Miscellaneous; Neurons - drug effects; Neurons - metabolism; Oxidopamine; Parkinson Disease, Secondary - chemically induced; Parkinson Disease, Secondary - pathology; Parkinson Disease, Secondary - therapy; Parkinson's disease; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects); Rats; Rats, Sprague-Dawley; Striatum; Substantia nigra; Substantia Nigra - metabolism; Substantia Nigra - pathology; Tyrosine 3-Monooxygenase - metabolism; Tyrosine hydroxylase; Vertebrates: nervous system and sense organs; Zusanli acupoint
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/j.neulet.2005.04.068

In this study, we investigated the effect of acupuncture at the Zusanli acupoint (ST36) on the nigrostriatal dopaminergic neuronal cell death in the rats with Parkinson's disease. Two weeks after unilateral injection of 6-hydroxydopamine (6-OHDA) into the striatum, an apomorphine-induced rotational behavior test showed significant rotational asymmetry in the rats with Parkinson's disease. Immunostaining for tyrosine hydroxylase demonstrated a dopaminergic neuronal loss in the substantia nigra and dopaminergic fiber loss in the striatum. Acupuncture at the ST36 for 14 days significantly inhibited rotational asymmetry in the rats with Parkinson's disease, and also protected against 6-OHDA-induced nigrostriatal dopaminergic neuronal loss. These effects of acupuncture were not observed for the non-acupoint (hip) acupuncture. The present study shows that acupuncture at the ST36 acupoint can be used as a useful strategy for the treatment of Parkinson's disease.