New approach for vitamin E extraction in rainbow trout flesh: Application in fish fed commercial and red seaweed‐supplemented diets

A vitamin E extraction method for rainbow trout flesh was optimized, validated, and applied in fish fed commercial and Gracilaria vermiculophylla‐supplemented diets. Five extraction methods were compared. Vitamers were analyzed by HPLC/DAD/fluorescence. A solid‐liquid extraction with n‐hexane, which...

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Bibliographic Details
Published in:European journal of lipid science and technology 2015-09, Vol.117 (9), p.1398-1405
Main Authors: Araújo, Mariana, Alves, Rita C, Pimentel, Filipa B, Costa, Anabela S.G, Fernandes, Telmo J.R, Valente, Luísa M.P, Rema, Paulo, Oliveira, M. Beatriz P.P
Format: Article
Language:English
Subjects:
Algae
alpha-tocopherol
aquaculture
diet
Extraction method
Feed
feed industry
Fish
fish feeds
fish health
fluorescence
gamma-tocopherol
Gracilaria
Gracilaria vermiculophylla
hexane
high performance liquid chromatography
information sources
lipids
macroalgae
meat quality
muscles
Oncorhynchus mykiss
Rainbow trout flesh
solvents
Trout
Vitamin E
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Summary:A vitamin E extraction method for rainbow trout flesh was optimized, validated, and applied in fish fed commercial and Gracilaria vermiculophylla‐supplemented diets. Five extraction methods were compared. Vitamers were analyzed by HPLC/DAD/fluorescence. A solid‐liquid extraction with n‐hexane, which showed the best performance, was optimized and validated. Among the eight vitamers, only α‐ and γ‐tocopherol were detected in muscle samples. The final method showed good linearity (>0.999), intra‐ (
ISSN:1438-7697
1438-9312
DOI:10.1002/ejlt.201400656