Mapping Major Replication Origins on the Rice Plastid DNA

To maintain and to differentiate into various plastid lineages, replication of the plastid DNA (ptDNA) and division of the plastid must take place. However, replication initiation of the ptDNA has been less understood. The present study describes identification of the initiation region (origin) of p...

Full description

Saved in:
Bibliographic Details
Published in:Plant Biotechnology 2002, Vol.19(1), pp.27-35
Main Authors: WANG, Ying, TAMURA, Kohya, SAITOH, Yasushi, SATO, Tadashi, HIDAKA, Soh, TSUTSUMI, Ken-ichi
Format: Article
Language:English
Subjects:
Oryza sativa
plastid DNA
replication
replication origin
rice
two-dimensional agarose gel electrophoresis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To maintain and to differentiate into various plastid lineages, replication of the plastid DNA (ptDNA) and division of the plastid must take place. However, replication initiation of the ptDNA has been less understood. The present study describes identification of the initiation region (origin) of ptDNA replication in the rice cultured cells. RNA-primed newly replicated DNA strands pulse-labeled with bromodeoxyuridine were isolated and size-fractionated. Locations of these nascent strands on the ptDNA determined the two major origin regions around the 3' region of each 23S rDNA in the inverted repeats (IRA and IRB). Two-dimensional agarose gel electrophoresis of the replication intermediates suggested that replication from each origin proceeds bidirectionally. This contrasted to replication by the double D-loop mechanism.
ISSN:1342-4580
1347-6114
DOI:10.5511/plantbiotechnology.19.27