method for bacteriocin quantification

Different aspects of the most commonly used assay methods in the study of bacteriocins were examined. The conditions under which extraction and incubation (including exposure time) take place were analysed, and several different formal models that are usually employed to calculate ID50 were compared...

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Bibliographic Details
Published in:Journal of applied microbiology 1999-12, Vol.87 (6), p.907-914
Main Authors: Cabo, M.L, Murado, M.A, Gonzalez, M.P, Pastoriza, L
Format: Article
Language:English
Subjects:
Animals
Bacteriocins - isolation & purification
Bacteriocins - pharmacology
Bacteriology
Biological and medical sciences
Culture Media
equations
exposure duration
Food Microbiology
Fundamental and applied biological sciences. Psychology
Hydrogen-Ion Concentration
Inhibitory Concentration 50
Lactococcus lactis - chemistry
Leuconostoc - drug effects
Leuconostoc - growth & development
Leuconostoc mesenteroides
Microbial Sensitivity Tests
Microbiology
microorganisms
Models, Biological
nisin
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Salmon - microbiology
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Summary:Different aspects of the most commonly used assay methods in the study of bacteriocins were examined. The conditions under which extraction and incubation (including exposure time) take place were analysed, and several different formal models that are usually employed to calculate ID50 were compared. As an alternative designed to overcome the problems which characterize the response of micro-organisms that are sensitive to bacteriocins, an operative procedure in a liquid medium and a modified re-parameterized logistic equation is proposed. When applied to the inhibition of Leuconostoc mesenteroides by nisin, the model allows an optimal experimental procedure to be defined.
ISSN:1364-5072
1365-2672
DOI:10.1046/j.1365-2672.1999.00942.x