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method for bacteriocin quantification

Different aspects of the most commonly used assay methods in the study of bacteriocins were examined. The conditions under which extraction and incubation (including exposure time) take place were analysed, and several different formal models that are usually employed to calculate ID50 were compared...

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Published in:	Journal of applied microbiology 1999-12, Vol.87 (6), p.907-914
Main Authors:	Cabo, M.L, Murado, M.A, Gonzalez, M.P, Pastoriza, L
Format:	Article
Language:	English
Subjects:	Animals Bacteriocins - isolation & purification Bacteriocins - pharmacology Bacteriology Biological and medical sciences Culture Media equations exposure duration Food Microbiology Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Inhibitory Concentration 50 Lactococcus lactis - chemistry Leuconostoc - drug effects Leuconostoc - growth & development Leuconostoc mesenteroides Microbial Sensitivity Tests Microbiology microorganisms Models, Biological nisin Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Salmon - microbiology
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description	Different aspects of the most commonly used assay methods in the study of bacteriocins were examined. The conditions under which extraction and incubation (including exposure time) take place were analysed, and several different formal models that are usually employed to calculate ID50 were compared. As an alternative designed to overcome the problems which characterize the response of micro-organisms that are sensitive to bacteriocins, an operative procedure in a liquid medium and a modified re-parameterized logistic equation is proposed. When applied to the inhibition of Leuconostoc mesenteroides by nisin, the model allows an optimal experimental procedure to be defined.
doi_str_mv	10.1046/j.1365-2672.1999.00942.x
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source	Alma/SFX Local Collection
subjects	Animals Bacteriocins - isolation & purification Bacteriocins - pharmacology Bacteriology Biological and medical sciences Culture Media equations exposure duration Food Microbiology Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Inhibitory Concentration 50 Lactococcus lactis - chemistry Leuconostoc - drug effects Leuconostoc - growth & development Leuconostoc mesenteroides Microbial Sensitivity Tests Microbiology microorganisms Models, Biological nisin Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Salmon - microbiology
title	method for bacteriocin quantification
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