Agrobacterium-mediated Transformation of Oenanthe javanica (Blume) DC. Plants

Transgenic plants of Oenanthe javanica (Blume) DC. ‘Shimane-Midori’ were obtained by Agrobacterium-mediated gene transfer. Embryogenic calli derived from petiole segments were infected with Agrobacterium tumefaciens strain EHA101, harboring a binary vector pIG121Hm that included genes for GUS, kanam...

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Bibliographic Details
Published in:Plant Biotechnology 2002, Vol.19(5), pp.365-368
Main Authors: ENDO, Ryuko, SHIRASAWA-SEO, Naomi, ADACHI, Yoko, KANAHAMA, Koki
Format: Article
Language:English
Subjects:
Agrobacterium tumefaciens
GUS assay
Oenanthe javanica
transformation
transgenic plant
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Summary:Transgenic plants of Oenanthe javanica (Blume) DC. ‘Shimane-Midori’ were obtained by Agrobacterium-mediated gene transfer. Embryogenic calli derived from petiole segments were infected with Agrobacterium tumefaciens strain EHA101, harboring a binary vector pIG121Hm that included genes for GUS, kanamycin resistance and hygromycin resistance. After four days of co-culture on MS medium containing 0.1mgl-1 2, 4-D, the infected calli were transferred onto hormone-free MS medium containing 500mgl-1 carbenicilin. Seven days after the start of hormone-free MS medium, the calli were transferred onto MS medium containing 50mgl-1 hygromycin for selection. Approximately four month after infection, seventy-one hygromycin-resistant plantlets were formed from about 4g calli. Expression of the GUS gene was confirmed by histochemical assay. The results of Southern hybridization analysis indicated that at least three independent transgenic plants were obtained, and all of them had a single copy of the GUS gene in their genomes.
ISSN:1342-4580
1347-6114
DOI:10.5511/plantbiotechnology.19.365