Organophosphonate metabolism by a moderately halophilic bacterial isolate

A Gram-negative halophile isolated from soil beneath a road gritting salt pile grew optimally at 10% (w/v) NaCl and was shown most likely to be Chromohalobacter marismortui or Pseudomonas beijerinckii on the basis of 16S rRNA analysis. The strain utilised phosphonoacetate, 2-aminoethyl-, 3-aminoprop...

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Bibliographic Details
Published in:FEMS microbiology letters 2000-05, Vol.186 (2), p.171-175
Main Authors: Hayes, Velma E.A, Ternan, Nigel G, McMullan, Geoffrey
Format: Article
Language:English
Subjects:
2-aminoethylphosphonate
2-aminoethylphosphonate:pyruvate
Biodegradation
Chromohalobacter marismortui
C–P lyase
Halophilic
Organophosphonate
organophosphonates
Pho regulon
Pseudomonas beijerinckii
rRNA 16S
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Summary:A Gram-negative halophile isolated from soil beneath a road gritting salt pile grew optimally at 10% (w/v) NaCl and was shown most likely to be Chromohalobacter marismortui or Pseudomonas beijerinckii on the basis of 16S rRNA analysis. The strain utilised phosphonoacetate, 2-aminoethyl-, 3-aminopropyl-, 4-aminobutyl-, methyl- and ethyl-phosphonates as phosphorus sources for growth. Differences were observed in the growth rate on different phosphonates and the range of phosphonates utilised at elevated NaCl concentrations, possibly as a result of differentially-induced transport mechanisms. An assay of cell-free extracts of 2-aminoethylphosphonate (2AEP) grown cells showed no detectable 2AEP:pyruvate aminotransferase or phosphonoacetaldehyde hydrolase activity.
ISSN:0378-1097
1574-6968
DOI:10.1016/S0378-1097(00)00136-1