2,4-Dichlorophenoxyacetic acid and kinetin in anther culture of cultivated and wild oats and their interspecific crosses: plant regeneration from A. sativa L

• Published in: Plant cell reports 1999-03, Vol.18 (7/8), p.582-588
• Main Authors: Kiviharju, E.M, Tauriainen, A.A
• Format: Article
• Language: English
• Subjects: 2,4-D; Anther and ovule culture, utilization for haploid production; anther culture; application rate; Avena sativa; Avena sterilis; Biological and medical sciences; Biotechnology; culture media; embryo (plant); Embryos; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; genotype; Genotypes; interspecific hybridization; kinetin; Methods. Procedures. Technologies; Oats; Plant cells and fungal cells; progeny; regenerative ability; somatic embryogenesis
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s002990050626

The effect of 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin was studied in anther culture of oat Avena sativa L., wild oat A. sterilis L. and progeny of crosses between them. A high 2,4-D concentration (5-6 mg l-1) increased embryo production in genotypes of both species and promoted plant regeneration in anther cultures of A. sterilis and A. sativa X A. sterilis progeny, while kinetin caused severe browning. However, a low concentration of kinetin was essential for initiation of regenerable embryos from anther culture of A. sativa cv. Kolbu: one green and one albino plant were produced. In addition, medium containing W14 salts gave higher regenerant recovery compared with medium containing Murashge and Skoog salts, when cross progeny were tested.