Cathepsin D in pancreatic acinar cells is implicated in cathepsin B and L degradation, but not in autophagic activity

Cathepsin D (CD) is the major lysosomal aspartic protease and is widely distributed in the cells of various mammalian tissues. CD participates in various physiological events such as regulation of programmed cell death, activation of enzymatic precursors, and metabolic degradation of intracellular p...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2016-01, Vol.469 (3), p.405-411
Main Authors: Mehanna, Sally, Suzuki, Chigure, Shibata, Masahiro, Sunabori, Takehiko, Imanaka, Takanobu, Araki, Kimi, Yamamura, Ken-ichi, Uchiyama, Yasuo, Ohmuraya, Masaki
Format: Article
Language:English
Subjects:
Acinar Cells - metabolism
Acinar Cells - pathology
Acute pancreatitis
Animals
Autophagy
Cathepsin B - metabolism
Cathepsin D
Cathepsin D - metabolism
Cathepsin L - metabolism
Cells, Cultured
Cysteine proteases
Mice
Mice, Inbred C57BL
Pancreatitis - metabolism
Pancreatitis - pathology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cathepsin D (CD) is the major lysosomal aspartic protease and is widely distributed in the cells of various mammalian tissues. CD participates in various physiological events such as regulation of programmed cell death, activation of enzymatic precursors, and metabolic degradation of intracellular proteins through macroautophagy. To investigate the role of CD in pancreatic acinar cells, which constitute the exocrine pancreas, we generated and examined mice specifically deficient for CD in pancreatic acinar cells. CD deficient mice showed normal pancreatic development and autophagic activity, although LC3-II, which is a marker of the autophagosome, accumulates in both physiological and pancreatitis conditions. Moreover, CD deficiency leads to accumulation of matured cathepsin B (CB) and cathepsin L (CL) which are members of the cysteine protease family. We therefore conclude that CD in pancreatic acinar cells is implicated in CB and CL degradation but not in autophagic activity.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2015.12.002