Development of a localized surface plasmon resonance-based gold nanobiosensor for the determination of prolactin hormone in human serum

• Published in: Analytical biochemistry 2016-02, Vol.495, p.32-36
• Main Authors: Faridli, Zahra, Mahani, Mohamad, Torkzadeh-Mahani, Masoud, Fasihi, Javad
• Format: Article
• Language: English
• Subjects: Antibodies - chemistry; Biosensing Techniques - instrumentation; Blood Chemical Analysis - instrumentation; Blood Chemical Analysis - methods; Dynamic Light Scattering; Gold - chemistry; Gold nanoparticles; Humans; Hydrogen-Ion Concentration; Immunoassay; Immunoassay - instrumentation; Label-free detection; Limit of Detection; Localized surface plasmon resonance; Metal Nanoparticles - chemistry; Nanobiosensor; Prolactin; Prolactin - blood; Surface Plasmon Resonance
• ISSN: 0003-2697; 1096-0309
• DOI: 10.1016/j.ab.2015.11.016

A localized surface plasmon resonance immunoassay has been developed to determine prolactin hormone in human serum samples. Gold nanoparticles were synthesized, and the probe was prepared by electrostatic adsorption of antibody on the surfaces of gold nanoparticles. The pH and the antibody-to-gold nanoparticle ratio, as the factors affecting the probe functions, were optimized. The constructed nanobiosensor was characterized by dynamic light scattering. The sensor was applied for the determination of prolactin antigen concentration based on the amount of localized surface plasmon resonance peak shift. A linear dynamic range of 1–40 ng ml−1, a detection limit of 0.8 ng ml−1, and sensitivity of 10 pg ml−1 were obtained. Finally, the nanobiosensor was applied for the determination of prolactin in human control serum sample.