Organized Aggregation of Porphyrins in Lipid Bilayers for Third Harmonic Generation Microscopy

Nonlinear optical microscopy has become a powerful tool for high‐resolution imaging of cellular and subcellular composition, morphology, and interactions because of its high spatial resolution, deep penetration, and low photo‐damage to tissue. Developing specific harmonic probes is essential for exp...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2015-11, Vol.54 (47), p.13928-13932
Main Authors: Cui, Liyang, Tokarz, Danielle, Cisek, Richard, Ng, Kenneth K., Wang, Fan, Chen, Juan, Barzda, Virginijus, Zheng, Gang
Format: Article
Language:English
Subjects:
Cell Line, Tumor
harmonic generation
Humans
Lipid Bilayers - chemistry
Microscopy
Microscopy - methods
multimodality imaging
nonlinear optical properties
organized aggregation
porphyrin nanoparticles
Porphyrins - chemistry
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Summary:Nonlinear optical microscopy has become a powerful tool for high‐resolution imaging of cellular and subcellular composition, morphology, and interactions because of its high spatial resolution, deep penetration, and low photo‐damage to tissue. Developing specific harmonic probes is essential for exploiting nonlinear microscopic imaging for biomedical applications. We report an organized aggregate of porphyrins (OAP) that formed within lipidic nanoparticles showing fingerprint spectroscopic properties, structure‐associated second harmonic generation, and superradiant third harmonic generation. The OAP facilitated harmonic microscopic imaging of living cells with significantly enhanced contrast. The structure‐dependent switch between harmonic (OAP‐intact) and fluorescence (OAP‐disrupted) generation enabled real‐time multi‐modality imaging of the cellular fate of nanoparticles. Robustly produced under various conditions and easily incorporated into pre‐formed lipid nanovesicles, OAP provides a biocompatible nanoplatform for harmonic imaging. The assembly and characterization of an organized aggregation of porphyrins (OAP) for nonlinear optical microscopy is reported. Its structure‐dependent photoproperty switch between harmonic (OAP‐intact) and fluorescence (OAP‐disrupted) generation permitted nanoparticle tracking in living cells. Green bars=porphyrin aggregates.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201506171