Construction of Metal-Ion-Free G‑quadruplex-Hemin DNAzyme and Its Application in S1 Nuclease Detection

In this work, a new kind of peroxidase-mimicking DNAzyme (G-quadruplex-hemin DNAzyme, G4-hemin) was constructed by using hemin-modified G-rich DNA (hemin-G-DNA). Experimental results demonstrated that the G-rich DNA can form a G-quadruplex structure by the inducement of terminally modified hemin, re...

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Bibliographic Details
Published in:ACS applied materials & interfaces 2016-01, Vol.8 (1), p.827-833
Main Authors: Wang, Zhaoyin, Zhao, Jian, Bao, Jianchun, Dai, Zhihui
Format: Article
Language:English
Subjects:
Animals
Cattle
Deoxyribonucleases - analysis
DNA, Catalytic - chemical synthesis
G-Quadruplexes
Hemin - chemical synthesis
Ions
Metals - chemistry
Peroxidase
Potassium
Spectrophotometry, Ultraviolet
Time Factors
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Summary:In this work, a new kind of peroxidase-mimicking DNAzyme (G-quadruplex-hemin DNAzyme, G4-hemin) was constructed by using hemin-modified G-rich DNA (hemin-G-DNA). Experimental results demonstrated that the G-rich DNA can form a G-quadruplex structure by the inducement of terminally modified hemin, rendering the assembly of hemin and G-quadruplex structure spontaneously and efficiently. As a result, G-hemin revealed higher peroxidase activity than traditional G-quadruplex/hemin DNAzyme (G4/hemin). Besides, different from G4/hemin, G4-hemin was constructed in one step without the participation of metal ions and adscititious hemin. Accordingly, the construction procedure was significantly simplified and the background signal from dissociative hemin was remarkably reduced. In a proof-of-concept trial, according to the colorimetric signals of G4-hemin, a novel biosensor for the detection of S1 nuclease activity was established, which provides a novel perspective for designing peroxidase-mimicking DNAzyme-based biosensors.
ISSN:1944-8244
1944-8252
DOI:10.1021/acsami.5b10165