Novel Isoform of the Hepatic Antimicrobial Peptide, Hepcidin (Hepc-CB1), from a Deep-Sea Fish, the Spinyjaw Greeneye Chlorophthalmus bicornis (Norman, 1939): Molecular Characterisation and Phylogeny

Hepcidin is cysteine-rich short peptide of innate immune system of fishes, equipped to perform prevention and proliferation of invading pathogens like bacteria and viruses by limiting iron availability and activating intracellular cascades. Hepcidins are diverse in teleost fishes, due to the varied...

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Bibliographic Details
Published in:Probiotics and antimicrobial proteins 2013-03, Vol.5 (1), p.1-7
Main Authors: Chaithanya, E. R, Philip, Rosamma, Sathyan, Naveen, Anil Kumar, P. R, Antony, Swapna P, Sanjeevan, V. N, Bright Singh, I. S
Format: Article
Language:English
Subjects:
Applied Microbiology
aquatic environment
bacteria
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Chlorophthalmus
evolutionary adaptation
gene duplication
hepcidin
innate immunity
marine fish
messenger RNA
Microbiology
Nutrition
pathogens
phylogeny
Protein Science
signal peptide
viruses
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Summary:Hepcidin is cysteine-rich short peptide of innate immune system of fishes, equipped to perform prevention and proliferation of invading pathogens like bacteria and viruses by limiting iron availability and activating intracellular cascades. Hepcidins are diverse in teleost fishes, due to the varied aquatic environments including exposure to pathogens, oxygenation and iron concentration. In the present study, we report a 87-amino acid (aa) preprohepcidin (Hepc-CB1) with a signal peptide of 24 aa, a prodomain of 39 aa and a bioactive mature peptide of 24 aa from the gill mRNA transcripts of the deep-sea fish spinyjaw greeneye, Chlorophthalmus bicornis. Molecular characterisation and phylogenetic analysis categorised the peptide to HAMP2-like group with a mature peptide of 2.53 kDa; a net positive charge (+3) and capacity to form β-hairpin-like structure configured by 8 conserved cysteines. The present work provides new insight into the mass gene duplication events and adaptive evolution of hepcidin isoforms with respect to environmental influences and positive Darwinian selection. This work reports a novel hepcidin isoform under the group HAMP2 from a non-acanthopterygian deep-sea fish, C. bicornis.
ISSN:1867-1306
1867-1314
DOI:10.1007/s12602-012-9120-0