Heartwood extract of Rhus verniciflua Stokes and its active constituent fisetin attenuate vasoconstriction through calcium-dependent mechanism in rat aorta

Rhus verniciflua Stokes (RVS) exert cardiovascular protective activity by promoting blood circulation, but its active ingredients and underlying mechanism have yet to be identified. This study investigated the vascular effects of RVS, focusing on vasoconstriction and smooth muscle Ca 2+ signaling. R...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2016-03, Vol.80 (3), p.493-500
Main Authors: Park, Jung-Min, Lee, Jun-Hyeong, Na, Chun-Soo, Lee, Dongho, Lee, Jin-Yong, Satoh, Masahiko, Lee, Moo-Yeol
Format: Article
Language:English
Subjects:
Animals
Aorta - cytology
Aorta - drug effects
Aorta - physiology
Calcium - metabolism
calcium signaling
Calcium Signaling - drug effects
Cells, Cultured
fisetin
Flavonoids - pharmacology
Male
Plant Extracts - pharmacology
Rats
Rats, Sprague-Dawley
Rhus - chemistry
Rhus verniciflua Stokes
vascular smooth muscle cells
vasoconstriction
Vasoconstriction - drug effects
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Summary:Rhus verniciflua Stokes (RVS) exert cardiovascular protective activity by promoting blood circulation, but its active ingredients and underlying mechanism have yet to be identified. This study investigated the vascular effects of RVS, focusing on vasoconstriction and smooth muscle Ca 2+ signaling. RVS heartwood extract attenuated contraction of aortic rings induced by the vasoconstrictors serotonin and phenylephrine, and inhibited the Ca 2+ signaling evoked by serotonin in vascular smooth muscle cells. Subsequent activity-guided fractionation identified fisetin as an active constituent exerting a Ca 2+ inhibitory effect. Fisetin could inhibit major Ca 2+ mobilization pathways including extracellular Ca 2+ influx mediated by the L-type voltage-gated Ca 2+ channel, Ca 2+ release from the intracellular store and store-operated Ca 2+ entry. In accordance with Ca 2+ inhibitory effect, fisetin attenuated vasoconstriction by serotonin and phenylephrine. These results suggest that the anticontractile effect, which is presumably mediated by inhibition of Ca 2+ signaling, may contribute to the improvement of blood circulation by RVS. Rhus verniciflua Stokes extract and its active constituent fisetin improve blood circulation by inhibiting smooth muscle Ca 2+ signaling and thereby attenuating vasoconstriction.
ISSN:0916-8451
1347-6947
DOI:10.1080/09168451.2015.1107464