Ultrasensitive Detection of Ebola Virus Oligonucleotide Based on Upconversion Nanoprobe/Nanoporous Membrane System

Ebola outbreaks are currently of great concern, and therefore, development of effective diagnosis methods is urgently needed. The key for lethal virus detection is high sensitivity, since early-stage detection of virus may increase the probability of survival. Here, we propose a luminescence scheme...

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Bibliographic Details
Published in:ACS nano 2016-01, Vol.10 (1), p.598-605
Main Authors: Tsang, Ming-Kiu, Ye, WeiWei, Wang, Guojing, Li, Jingming, Yang, Mo, Hao, Jianhua
Format: Article
Language:English
Subjects:
Barium Compounds - chemistry
Biological Assay
Ebolavirus - genetics
Ebolavirus - isolation & purification
Europium - chemistry
Fluorescence Resonance Energy Transfer
Fluorides
Gold - chemistry
Hemorrhagic Fever, Ebola - diagnosis
Hemorrhagic Fever, Ebola - virology
Humans
Limit of Detection
Membranes, Artificial
Metal Nanoparticles - chemistry
Oligonucleotides - chemical synthesis
Oligonucleotides - chemistry
Porosity
Ytterbium - chemistry
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Summary:Ebola outbreaks are currently of great concern, and therefore, development of effective diagnosis methods is urgently needed. The key for lethal virus detection is high sensitivity, since early-stage detection of virus may increase the probability of survival. Here, we propose a luminescence scheme of assay consisting of BaGdF5:Yb/Er upconversion nanoparticles (UCNPs) conjugated with oligonucleotide probe and gold nanoparticles (AuNPs) linked with target Ebola virus oligonucleotide. As a proof of concept, a homogeneous assay was fabricated and tested, yielding a detection limit at picomolar level. The luminescence resonance energy transfer is ascribed to the spectral overlapping of upconversion luminescence and the absorption characteristics of AuNPs. Moreover, we anchored the UCNPs and AuNPs on a nanoporous alumina (NAAO) membrane to form a heterogeneous assay. Importantly, the detection limit was greatly improved, exhibiting a remarkable value at the femtomolar level. The enhancement is attributed to the increased light–matter interaction throughout the nanopore walls of the NAAO membrane. The specificity test suggested that the nanoprobes were specific to Ebola virus oligonucleotides. The strategy combining UCNPs, AuNPs, and NAAO membrane provides new insight into low-cost, rapid, and ultrasensitive detection of different diseases. Furthermore, we explored the feasibility of clinical application by using inactivated Ebola virus samples. The detection results showed great potential of our heterogeneous design for practical application.
ISSN:1936-0851
1936-086X
DOI:10.1021/acsnano.5b05622