Carboxylic Carbon Quantum Dots as a Fluorescent Sensing Platform for DNA Detection

• Published in: ACS applied materials & interfaces 2016-01, Vol.8 (3), p.1951-1957
• Main Authors: Loo, Adeline Huiling, Sofer, Zdenek, Bouša, Daniel, Ulbrich, Pavel, Bonanni, Alessandra, Pumera, Martin
• Format: Article
• Language: English
• Subjects: Calibration; Carbon - chemistry; Carboxylic Acids - chemistry; DNA - analysis; Fluorescent Dyes - chemistry; Photoelectron Spectroscopy; Quantum Dots - chemistry; Spectrometry, Fluorescence; Spectroscopy, Fourier Transform Infrared
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/acsami.5b10160

The demand for simple, sensitive, affordable, and selective DNA biosensors is ubiquitous, due to the important role that DNA detection performs in the areas of disease diagnostics, environment monitoring, and food safety. A novel application of carboxylic carbon quantum dots (cCQD) is highlighted in this study. Herein, cCQD function as a nanoquencher in the detection of nucleic acid based on a homogeneous fluorescent assay. To that purpose, the performance of two types of cCQD, namely, citric acid QD and malic acid QD, is evaluated. The principle behind the sensing of nucleic acid lies in the different propensity of single-stranded DNA and double-stranded DNA to adsorb onto the surface of cCQD. For both types of cCQD, a superior range of detection of at least 3 orders of magnitude is achieved, and the potential to distinguish single-base mismatch is also exhibited. These findings are anticipated to provide valuable insights on the employment of cCQD for the fabrication of future DNA biosensors.