The Phenotypic and Functional Features of Human M2 Macrophages Generated Under Low Serum Conditions

The phenotypic and functional features of human M2 macrophages, in particular, their immunosuppressive activity, can considerably vary depending on M2 polarizing stimulus. This study was aimed at the investigation of cytokine production and pro‐apoptogenic/inhibitory molecule expression in macrophag...

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Bibliographic Details
Published in:Scandinavian journal of immunology 2016-02, Vol.83 (2), p.151-159
Main Authors: Sakhno, L. V., Shevela, E. Ya, Tikhonova, M. A., Ostanin, A. A., Chernykh, E. R.
Format: Article
Language:English
Subjects:
Adult
Apoptosis
Apoptosis Regulatory Proteins - immunology
Cell Differentiation - drug effects
Cell Differentiation - immunology
Cell Proliferation
Chemokines - biosynthesis
Culture Media
Culture Media, Serum-Free
Cytokines - biosynthesis
Cytotoxicity, Immunologic
Female
Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology
Humans
In Vitro Techniques
Lectins, C-Type - metabolism
Macrophages - cytology
Macrophages - drug effects
Macrophages - immunology
Male
Mannose-Binding Lectins - metabolism
Phenotype
Receptors, Cell Surface - metabolism
T-Lymphocytes - immunology
TNF-Related Apoptosis-Inducing Ligand - metabolism
Young Adult
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Summary:The phenotypic and functional features of human M2 macrophages, in particular, their immunosuppressive activity, can considerably vary depending on M2 polarizing stimulus. This study was aimed at the investigation of cytokine production and pro‐apoptogenic/inhibitory molecule expression in macrophages generated with GM‐CSF using either standard conditions (M1) or deficiency of serum/growth factors (M2‐LS cells). In contrast to M1, M2‐LS cells were characterized by an enhanced content of CD206+, B7‐H1+, FasL+ and TRAIL+ cells along with a decreased production of IFN‐γ, IL‐5, IL‐6, IL‐13, TNF‐α, IL‐17 and MCP‐1. In addition, M2‐LS exhibited a lower T cell stimulatory activity in MLC that was associated with the higher numbers of apoptotic and the lower numbers of proliferating T cells. B7‐H1 plays a key role in M2‐LS‐mediated cytotoxic effects as the neutralization of B7‐H1 reduces the apoptosis‐inducing activity of M2‐LS, while the blocking of CD206 and TRAIL reduces the cytostatic activity of M2 macrophages.
ISSN:0300-9475
1365-3083
DOI:10.1111/sji.12401