Seventy-five EST-linked Atlantic salmon (Salmo salar L.) microsatellite markers and their cross-amplification in five salmonid species

An Atlantic salmon (Salmo salar L.) expressed sequence tag (EST) database consisting of 58 146 ESTs was screened for microsatellite sequences. Subsequent development of 75 polymorphic EST‐associated microsatellite markers in this species is described together with cross‐species amplification results...

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Bibliographic Details
Published in:Molecular ecology notes 2005-06, Vol.5 (2), p.282-288
Main Authors: Vasemagi, A, Nilsson, J, Primmer, C.R
Format: Article
Language:English
Subjects:
alleles
alleles per locus
cDNA
Coregonus
Coregonus lavaretus
cross-species amplification
DNA primers
EST
expressed sequence tag
expressed sequence tags
genetic markers
heterozygosity
loci
microsatellite
microsatellite repeats
molecular sequence data
nucleotide sequences
Oncorhynchus mykiss
polymerase chain reaction
polymorphic loci
Salmo salar
Salmo trutta
salmon
salmonidae
Salvelinus alpinus
Thymallus
Thymallus thymallus
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Summary:An Atlantic salmon (Salmo salar L.) expressed sequence tag (EST) database consisting of 58 146 ESTs was screened for microsatellite sequences. Subsequent development of 75 polymorphic EST‐associated microsatellite markers in this species is described together with cross‐species amplification results of 133 gene‐associated tandem repeat markers in five salmonid species (Salmo trutta, Oncorhynchus mykiss, Salvelinus aplinus, Thymallus thymallus, Coregonus lavaretus). The number of alleles among EST‐linked microsatellites in Atlantic salmon ranged from two to 41 with an average of 12 alleles per locus. Cross‐species amplification resulted in detection of a total of 111 polymorphic locus‐species combinations (12–32 loci per species).
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2005.00902.x