Development and characterization of novel tetra-, tri-, and dinucleotide microsatellite markers in rainbow trout (Oncorhynchus mykiss)

We discuss the development and characterization of 40 polymorphic rainbow trout (Oncorhynchus mykiss) microsatellite loci. We used enriched libraries to isolate 14 dinucleotide, seven trinucleodide, eight compound di/tetranucleotide, and 11 tetranucleotide loci. These markers will be useful for sele...

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Bibliographic Details
Published in:Molecular ecology notes 2005-06, Vol.5 (2), p.278-281
Main Authors: Spies, I.B, Brasier, D.J, O'Reilly, P.T.L, Seamons, T.R, Bentzen, P
Format: Article
Language:English
Subjects:
alleles
alleles per locus
DNA primers
enrichment
genetic markers
genome mapping
heterozygosity
loci
microsatellite repeats
microsatellites
molecular sequence data
nucleotide sequences
Oncorhynchus mykiss
polymerase chain reaction
polymorphic loci
population genetics
rainbow trout
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Summary:We discuss the development and characterization of 40 polymorphic rainbow trout (Oncorhynchus mykiss) microsatellite loci. We used enriched libraries to isolate 14 dinucleotide, seven trinucleodide, eight compound di/tetranucleotide, and 11 tetranucleotide loci. These markers will be useful for selective breeding via marker‐assisted selection, population genetics studies, parentage analysis, and have already been used for genome mapping.
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2005.00900.x