Magnetic Nanoclusters Exhibiting Protein-Activated Near-Infrared Fluorescence

Composite nanoclusters with chemical, magnetic, and biofunctionality offer broad opportunities for targeted cellular imaging. A key challenge is to load a high degree of targeting, imaging, and therapeutic functionality onto stable metal-oxide nanoparticles. Here we report a route for producing magn...

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Bibliographic Details
Published in:ACS nano 2013-01, Vol.7 (1), p.203-213
Main Authors: Daniele, Michael A, Shaughnessy, Margaret L, Roeder, Ryan, Childress, Anthony, Bandera, Yuriy P, Foulger, Stephen
Format: Article
Language:English
Subjects:
Cellular
Chemical compounds
Coprecipitation
Fluorescence
Imaging
Magnetite Nanoparticles - chemistry
Materials Testing
Metal oxides
Nanoparticles
Nanostructure
Proteins - chemistry
Serum albumin
Spectroscopy, Near-Infrared - methods
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Summary:Composite nanoclusters with chemical, magnetic, and biofunctionality offer broad opportunities for targeted cellular imaging. A key challenge is to load a high degree of targeting, imaging, and therapeutic functionality onto stable metal-oxide nanoparticles. Here we report a route for producing magnetic nanoclusters (MNCs) with alkyne surface functionality that can be utilized as multimodal imaging probes. We form MNCs composed of magnetic Fe3O4 nanoparticles and poly(acrylic acid-co-propargyl acrylate) by the co-precipitation of iron salts in the presence of copolymer stabilizers. The MNCs were surface-modified with near-infrared (NIR) emitting fluorophore used in photodynamic therapy, an azide-modified indocyanine green. The fluorophores engaged and complexed with bovine serum albumin, forming an extended coverage of serum proteins on the MNCs. These proteins isolated indocyanine green fluorophores from the aqueous environment and induced an effective “turn-on” of NIR emission.
ISSN:1936-0851
1936-086X
DOI:10.1021/nn3037368