Two-Color DNA Nanoprobe of Intracellular Dynamics

We have developed a correlation microscopy technique to follow the dynamics of quantum dot labeled DNA within living cells. The temporal correlation functions of the labels reflect the fluctuations of the DNA nanoprobe as a result of its interactions with the cellular environment. They provide a sen...

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Bibliographic Details
Published in:Nano letters 2012-05, Vol.12 (5), p.2515-2519
Main Authors: Milstein, Joshua N, Chu, Mike, Raghunathan, Krishnan, Meiners, Jens-Christian
Format: Article
Language:English
Subjects:
Applied sciences
Biomedical materials
Color
Correlation
Cross-disciplinary physics: materials science
rheology
Deoxyribonucleic acid
DNA Probes
Dynamics
Electronics
Exact sciences and technology
Fluctuation
General equipment and techniques
In vivo testing
In vivo tests
Instruments, apparatus, components and techniques common to several branches of physics and astronomy
Materials science
Microscopy
Molecular electronics, nanoelectronics
Nanocrystalline materials
Nanoscale materials and structures: fabrication and characterization
Nanostructure
Nanotechnology
Physics
Quantum dots
Semiconductor electronics. Microelectronics. Optoelectronics. Solid state devices
Sensors (chemical, optical, electrical, movement, gas, etc.)
remote sensing
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Summary:We have developed a correlation microscopy technique to follow the dynamics of quantum dot labeled DNA within living cells. The temporal correlation functions of the labels reflect the fluctuations of the DNA nanoprobe as a result of its interactions with the cellular environment. They provide a sensitive measure for the length of the probe on the scale of a persistence length (∼50 nm) and reveal strong nonthermal dynamics of the cell. These results pave the way for dynamic observations of DNA conformational changes in vivo.
ISSN:1530-6984
1530-6992
DOI:10.1021/nl300683p