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Two-Color DNA Nanoprobe of Intracellular Dynamics
We have developed a correlation microscopy technique to follow the dynamics of quantum dot labeled DNA within living cells. The temporal correlation functions of the labels reflect the fluctuations of the DNA nanoprobe as a result of its interactions with the cellular environment. They provide a sen...
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| Published in: | Nano letters 2012-05, Vol.12 (5), p.2515-2519 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-a411t-b5e6a9cb35c1635bf3e4524e100e04c00b01b605ff3a7abc492a76815ee955453 |
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| cites | cdi_FETCH-LOGICAL-a411t-b5e6a9cb35c1635bf3e4524e100e04c00b01b605ff3a7abc492a76815ee955453 |
| container_end_page | 2519 |
| container_issue | 5 |
| container_start_page | 2515 |
| container_title | Nano letters |
| container_volume | 12 |
| creator | Milstein, Joshua N Chu, Mike Raghunathan, Krishnan Meiners, Jens-Christian |
| description | We have developed a correlation microscopy technique to follow the dynamics of quantum dot labeled DNA within living cells. The temporal correlation functions of the labels reflect the fluctuations of the DNA nanoprobe as a result of its interactions with the cellular environment. They provide a sensitive measure for the length of the probe on the scale of a persistence length (∼50 nm) and reveal strong nonthermal dynamics of the cell. These results pave the way for dynamic observations of DNA conformational changes in vivo. |
| doi_str_mv | 10.1021/nl300683p |
| format | article |
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These results pave the way for dynamic observations of DNA conformational changes in vivo.</description><subject>Applied sciences</subject><subject>Biomedical materials</subject><subject>Color</subject><subject>Correlation</subject><subject>Cross-disciplinary physics: materials science; rheology</subject><subject>Deoxyribonucleic acid</subject><subject>DNA Probes</subject><subject>Dynamics</subject><subject>Electronics</subject><subject>Exact sciences and technology</subject><subject>Fluctuation</subject><subject>General equipment and techniques</subject><subject>In vivo testing</subject><subject>In vivo tests</subject><subject>Instruments, apparatus, components and techniques common to several branches of physics and astronomy</subject><subject>Materials science</subject><subject>Microscopy</subject><subject>Molecular electronics, nanoelectronics</subject><subject>Nanocrystalline materials</subject><subject>Nanoscale materials and structures: fabrication and characterization</subject><subject>Nanostructure</subject><subject>Nanotechnology</subject><subject>Physics</subject><subject>Quantum dots</subject><subject>Semiconductor electronics. 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| fulltext | fulltext |
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| ispartof | Nano letters, 2012-05, Vol.12 (5), p.2515-2519 |
| issn | 1530-6984 1530-6992 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1762050707 |
| source | American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list) |
| subjects | Applied sciences Biomedical materials Color Correlation Cross-disciplinary physics: materials science rheology Deoxyribonucleic acid DNA Probes Dynamics Electronics Exact sciences and technology Fluctuation General equipment and techniques In vivo testing In vivo tests Instruments, apparatus, components and techniques common to several branches of physics and astronomy Materials science Microscopy Molecular electronics, nanoelectronics Nanocrystalline materials Nanoscale materials and structures: fabrication and characterization Nanostructure Nanotechnology Physics Quantum dots Semiconductor electronics. Microelectronics. Optoelectronics. Solid state devices Sensors (chemical, optical, electrical, movement, gas, etc.) remote sensing |
| title | Two-Color DNA Nanoprobe of Intracellular Dynamics |
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