Highly Loaded Hemoglobin Spheres as Promising Artificial Oxygen Carriers

Seeking safe and effective artificial blood substitutes based on hemoglobin (Hb) as oxygen carriers is an important topic. A significant challenge is to enhance the loading content of Hb in a well-defined structure. Here we report a facile and controllable avenue to fabricate Hb spheres with a high...

Full description

Saved in:
Bibliographic Details
Published in:ACS nano 2012-08, Vol.6 (8), p.6897-6904
Main Authors: Duan, Li, Yan, Xuehai, Wang, Anhe, Jia, Yi, Li, Junbai
Format: Article
Language:English
Subjects:
Assembly
Blood substitutes
Blood Substitutes - chemical synthesis
Calcium carbonate
Carriers
Covalence
Crystallization - methods
Density
Hemoglobin
Hemoglobins - chemistry
Hemoglobins - ultrastructure
Materials Testing
Nanocapsules - chemistry
Nanocapsules - ultrastructure
Oxygen - chemistry
Particle Size
Polyethylene glycol
Protein Binding
Strategy
Surface Properties
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Seeking safe and effective artificial blood substitutes based on hemoglobin (Hb) as oxygen carriers is an important topic. A significant challenge is to enhance the loading content of Hb in a well-defined structure. Here we report a facile and controllable avenue to fabricate Hb spheres with a high loading content by templating decomposable porous CaCO3 particles in collaboration with covalent layer-by-layer assembly technique. The surface of the Hb spheres was further chemically modified by biocompatible polyethylene glycol to protect and stabilize the system. Multiple characterization techniques were employed to reveal the loading and density of Hb in an individual CaCO3 particle. The results demonstrate that the strategy developed in this work is effective and flexible for construction of the highly loaded Hb spheres. More importantly, such Hb spheres retain their carrying–releasing oxygen function. It may thus have great potential to develop Hb spheres with highly loaded content as realistic artificial blood substitutes in the future.
ISSN:1936-0851
1936-086X
DOI:10.1021/nn301735u