Probing Rotational and Translational Diffusion of Nanodoublers in Living Cells on Microsecond Time Scales

Nonlinear microscopes have seen an increase in popularity in the life sciences due to their molecular and structural specificity, high resolution, large penetration depth, and volumetric imaging capability. Nonetheless, the inherently weak optical signals demand long exposure times for live cell ima...

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Bibliographic Details
Published in:Nano letters 2014-05, Vol.14 (5), p.2552-2557
Main Authors: Macias-Romero, Carlos, Didier, Marie E. P, Zubkovs, Vitalijs, Delannoy, Lucas, Dutto, Fabrizia, Radenovic, Aleksandra, Roke, Sylvie
Format: Article
Language:English
Subjects:
Biological Science Disciplines - methods
Cellular
Condensed matter: structure, mechanical and thermal properties
Diffusion
Diffusion in nanoscale solids
Diffusion in solids
Epithelial Cells - ultrastructure
Exact sciences and technology
Humans
Illumination
Imaging
Nanoparticles - chemistry
Nanostructure
Nonlinear dynamics
Nonlinearity
Physics
Rotational
Transport properties of condensed matter (nonelectronic)
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Summary:Nonlinear microscopes have seen an increase in popularity in the life sciences due to their molecular and structural specificity, high resolution, large penetration depth, and volumetric imaging capability. Nonetheless, the inherently weak optical signals demand long exposure times for live cell imaging. Here, by modifying the optical layout and illumination parameters, we can follow the rotation and translation of noncentrosymetric crystalline particles, or nanodoublers, with 50 μs acquisition times in living cells. The rotational diffusion can be derived from variations in the second harmonic intensity that originates from the rotation of the nanodoubler crystal axis. We envisage that by capitalizing on the biocompatibility, functionalizability, stability, and nondestructive optical response of the nanodoublers, novel insights on cellular dynamics are within reach.
ISSN:1530-6984
1530-6992
DOI:10.1021/nl500356u