Loading…

Ceria Nanoparticles Stabilized by Organic Surface Coatings Activate the Lysosome-Autophagy System and Enhance Autophagic Clearance

Cerium oxide nanoparticles (nanoceria) are widely used in a variety of industrial applications including UV filters and catalysts. The expanding commercial scale production and use of ceria nanoparticles have inevitably increased the risk of release of nanoceria into the environment as well as the r...

Full description

Saved in:
Bibliographic Details
Published in:ACS nano 2014-10, Vol.8 (10), p.10328-10342
Main Authors: Song, Wensi, Soo Lee, Seung, Savini, Marzia, Popp, Lauren, Colvin, Vicki L, Segatori, Laura
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-a414t-ac707fc5f309024c3815fd6f2a99b41947a3534ef07bec472c15c22df110d0fa3
cites cdi_FETCH-LOGICAL-a414t-ac707fc5f309024c3815fd6f2a99b41947a3534ef07bec472c15c22df110d0fa3
container_end_page 10342
container_issue 10
container_start_page 10328
container_title ACS nano
container_volume 8
creator Song, Wensi
Soo Lee, Seung
Savini, Marzia
Popp, Lauren
Colvin, Vicki L
Segatori, Laura
description Cerium oxide nanoparticles (nanoceria) are widely used in a variety of industrial applications including UV filters and catalysts. The expanding commercial scale production and use of ceria nanoparticles have inevitably increased the risk of release of nanoceria into the environment as well as the risk of human exposure. The use of nanoceria in biomedical applications is also being currently investigated because of its recently characterized antioxidative properties. In this study, we investigated the impact of ceria nanoparticles on the lysosome-autophagy system, the main catabolic pathway that is activated in mammalian cells upon internalization of exogenous material. We tested a battery of ceria nanoparticles functionalized with different types of biocompatible coatings (N-acetylglucosamine, polyethylene glycol and polyvinylpyrrolidone) expected to have minimal effect on lysosomal integrity and function. We found that ceria nanoparticles promote activation of the transcription factor EB, a master regulator of lysosomal function and autophagy, and induce upregulation of genes of the lysosome-autophagy system. We further show that the array of differently functionalized ceria nanoparticles tested in this study enhance autophagic clearance of proteolipid aggregates that accumulate as a result of inefficient function of the lysosome-autophagy system. This study provides a mechanistic understanding of the interaction of ceria nanoparticles with the lysosome-autophagy system and demonstrates that ceria nanoparticles are activators of autophagy and promote clearance of autophagic cargo. These results provide insights for the use of nanoceria in biomedical applications, including drug delivery. These findings will also inform the design of engineered nanoparticles with safe and precisely controlled impact on the environment and the design of nanotherapeutics for the treatment of diseases with defective autophagic function and accumulation of lysosomal storage material.
doi_str_mv 10.1021/nn505073u
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1762057293</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1762057293</sourcerecordid><originalsourceid>FETCH-LOGICAL-a414t-ac707fc5f309024c3815fd6f2a99b41947a3534ef07bec472c15c22df110d0fa3</originalsourceid><addsrcrecordid>eNqFkU1v1DAQhi0EomXhwB9AviDRQ8Afcbw5rqJSkFb0sCBxiybOeNdVYi-2gxSO_HJStt0TEiePPM88h_cl5DVn7zkT_IP3iimm5fSEXPJaVgVbV9-fnmfFL8iLlO4YU3qtq-fkQijJVaXUJfndYHRAv4APR4jZmQET3WXo3OB-YU-7md7GPXhn6G6KFgzSJkB2fp_oxmT3EzLSfEC6nVNIYcRiM-VwPMB-prs5ZRwp-J5e-wP45fZxueiaASHef74kzywMCV89vCvy7eP11-ZTsb29-dxstgWUvMwFGM20NcpKVjNRGrnmyvaVFVDXXcnrUoNUskTLdIem1MJwZYToLeesZxbkirw7eY8x_Jgw5XZ0yeAwgMcwpZbrSiwJiVr-H634mpd_M1-RqxNqYkgpom2P0Y0Q55az9r6d9tzOwr550E7diP2ZfKxjAd6eADCpvQtT9Esg_xD9AQyml2k</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1618140507</pqid></control><display><type>article</type><title>Ceria Nanoparticles Stabilized by Organic Surface Coatings Activate the Lysosome-Autophagy System and Enhance Autophagic Clearance</title><source>American Chemical Society:Jisc Collections:American Chemical Society Read &amp; Publish Agreement 2022-2024 (Reading list)</source><creator>Song, Wensi ; Soo Lee, Seung ; Savini, Marzia ; Popp, Lauren ; Colvin, Vicki L ; Segatori, Laura</creator><creatorcontrib>Song, Wensi ; Soo Lee, Seung ; Savini, Marzia ; Popp, Lauren ; Colvin, Vicki L ; Segatori, Laura</creatorcontrib><description>Cerium oxide nanoparticles (nanoceria) are widely used in a variety of industrial applications including UV filters and catalysts. The expanding commercial scale production and use of ceria nanoparticles have inevitably increased the risk of release of nanoceria into the environment as well as the risk of human exposure. The use of nanoceria in biomedical applications is also being currently investigated because of its recently characterized antioxidative properties. In this study, we investigated the impact of ceria nanoparticles on the lysosome-autophagy system, the main catabolic pathway that is activated in mammalian cells upon internalization of exogenous material. We tested a battery of ceria nanoparticles functionalized with different types of biocompatible coatings (N-acetylglucosamine, polyethylene glycol and polyvinylpyrrolidone) expected to have minimal effect on lysosomal integrity and function. We found that ceria nanoparticles promote activation of the transcription factor EB, a master regulator of lysosomal function and autophagy, and induce upregulation of genes of the lysosome-autophagy system. We further show that the array of differently functionalized ceria nanoparticles tested in this study enhance autophagic clearance of proteolipid aggregates that accumulate as a result of inefficient function of the lysosome-autophagy system. This study provides a mechanistic understanding of the interaction of ceria nanoparticles with the lysosome-autophagy system and demonstrates that ceria nanoparticles are activators of autophagy and promote clearance of autophagic cargo. These results provide insights for the use of nanoceria in biomedical applications, including drug delivery. These findings will also inform the design of engineered nanoparticles with safe and precisely controlled impact on the environment and the design of nanotherapeutics for the treatment of diseases with defective autophagic function and accumulation of lysosomal storage material.</description><identifier>ISSN: 1936-0851</identifier><identifier>EISSN: 1936-086X</identifier><identifier>DOI: 10.1021/nn505073u</identifier><identifier>PMID: 25315655</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Animals ; Autophagy ; Biomedical materials ; Cell Line ; Cerium - chemistry ; Cerium oxide ; Clearances ; Coatings ; Diseases ; Humans ; Lysosomes - chemistry ; Metal Nanoparticles - chemistry ; Microscopy, Electron, Transmission ; Nanoparticles ; Nanostructure ; Risk ; Surface Properties</subject><ispartof>ACS nano, 2014-10, Vol.8 (10), p.10328-10342</ispartof><rights>Copyright © 2014 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a414t-ac707fc5f309024c3815fd6f2a99b41947a3534ef07bec472c15c22df110d0fa3</citedby><cites>FETCH-LOGICAL-a414t-ac707fc5f309024c3815fd6f2a99b41947a3534ef07bec472c15c22df110d0fa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25315655$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Song, Wensi</creatorcontrib><creatorcontrib>Soo Lee, Seung</creatorcontrib><creatorcontrib>Savini, Marzia</creatorcontrib><creatorcontrib>Popp, Lauren</creatorcontrib><creatorcontrib>Colvin, Vicki L</creatorcontrib><creatorcontrib>Segatori, Laura</creatorcontrib><title>Ceria Nanoparticles Stabilized by Organic Surface Coatings Activate the Lysosome-Autophagy System and Enhance Autophagic Clearance</title><title>ACS nano</title><addtitle>ACS Nano</addtitle><description>Cerium oxide nanoparticles (nanoceria) are widely used in a variety of industrial applications including UV filters and catalysts. The expanding commercial scale production and use of ceria nanoparticles have inevitably increased the risk of release of nanoceria into the environment as well as the risk of human exposure. The use of nanoceria in biomedical applications is also being currently investigated because of its recently characterized antioxidative properties. In this study, we investigated the impact of ceria nanoparticles on the lysosome-autophagy system, the main catabolic pathway that is activated in mammalian cells upon internalization of exogenous material. We tested a battery of ceria nanoparticles functionalized with different types of biocompatible coatings (N-acetylglucosamine, polyethylene glycol and polyvinylpyrrolidone) expected to have minimal effect on lysosomal integrity and function. We found that ceria nanoparticles promote activation of the transcription factor EB, a master regulator of lysosomal function and autophagy, and induce upregulation of genes of the lysosome-autophagy system. We further show that the array of differently functionalized ceria nanoparticles tested in this study enhance autophagic clearance of proteolipid aggregates that accumulate as a result of inefficient function of the lysosome-autophagy system. This study provides a mechanistic understanding of the interaction of ceria nanoparticles with the lysosome-autophagy system and demonstrates that ceria nanoparticles are activators of autophagy and promote clearance of autophagic cargo. These results provide insights for the use of nanoceria in biomedical applications, including drug delivery. These findings will also inform the design of engineered nanoparticles with safe and precisely controlled impact on the environment and the design of nanotherapeutics for the treatment of diseases with defective autophagic function and accumulation of lysosomal storage material.</description><subject>Animals</subject><subject>Autophagy</subject><subject>Biomedical materials</subject><subject>Cell Line</subject><subject>Cerium - chemistry</subject><subject>Cerium oxide</subject><subject>Clearances</subject><subject>Coatings</subject><subject>Diseases</subject><subject>Humans</subject><subject>Lysosomes - chemistry</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Microscopy, Electron, Transmission</subject><subject>Nanoparticles</subject><subject>Nanostructure</subject><subject>Risk</subject><subject>Surface Properties</subject><issn>1936-0851</issn><issn>1936-086X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNqFkU1v1DAQhi0EomXhwB9AviDRQ8Afcbw5rqJSkFb0sCBxiybOeNdVYi-2gxSO_HJStt0TEiePPM88h_cl5DVn7zkT_IP3iimm5fSEXPJaVgVbV9-fnmfFL8iLlO4YU3qtq-fkQijJVaXUJfndYHRAv4APR4jZmQET3WXo3OB-YU-7md7GPXhn6G6KFgzSJkB2fp_oxmT3EzLSfEC6nVNIYcRiM-VwPMB-prs5ZRwp-J5e-wP45fZxueiaASHef74kzywMCV89vCvy7eP11-ZTsb29-dxstgWUvMwFGM20NcpKVjNRGrnmyvaVFVDXXcnrUoNUskTLdIem1MJwZYToLeesZxbkirw7eY8x_Jgw5XZ0yeAwgMcwpZbrSiwJiVr-H634mpd_M1-RqxNqYkgpom2P0Y0Q55az9r6d9tzOwr550E7diP2ZfKxjAd6eADCpvQtT9Esg_xD9AQyml2k</recordid><startdate>20141028</startdate><enddate>20141028</enddate><creator>Song, Wensi</creator><creator>Soo Lee, Seung</creator><creator>Savini, Marzia</creator><creator>Popp, Lauren</creator><creator>Colvin, Vicki L</creator><creator>Segatori, Laura</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QQ</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20141028</creationdate><title>Ceria Nanoparticles Stabilized by Organic Surface Coatings Activate the Lysosome-Autophagy System and Enhance Autophagic Clearance</title><author>Song, Wensi ; Soo Lee, Seung ; Savini, Marzia ; Popp, Lauren ; Colvin, Vicki L ; Segatori, Laura</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a414t-ac707fc5f309024c3815fd6f2a99b41947a3534ef07bec472c15c22df110d0fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Autophagy</topic><topic>Biomedical materials</topic><topic>Cell Line</topic><topic>Cerium - chemistry</topic><topic>Cerium oxide</topic><topic>Clearances</topic><topic>Coatings</topic><topic>Diseases</topic><topic>Humans</topic><topic>Lysosomes - chemistry</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Microscopy, Electron, Transmission</topic><topic>Nanoparticles</topic><topic>Nanostructure</topic><topic>Risk</topic><topic>Surface Properties</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Song, Wensi</creatorcontrib><creatorcontrib>Soo Lee, Seung</creatorcontrib><creatorcontrib>Savini, Marzia</creatorcontrib><creatorcontrib>Popp, Lauren</creatorcontrib><creatorcontrib>Colvin, Vicki L</creatorcontrib><creatorcontrib>Segatori, Laura</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Ceramic Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>ACS nano</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Song, Wensi</au><au>Soo Lee, Seung</au><au>Savini, Marzia</au><au>Popp, Lauren</au><au>Colvin, Vicki L</au><au>Segatori, Laura</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ceria Nanoparticles Stabilized by Organic Surface Coatings Activate the Lysosome-Autophagy System and Enhance Autophagic Clearance</atitle><jtitle>ACS nano</jtitle><addtitle>ACS Nano</addtitle><date>2014-10-28</date><risdate>2014</risdate><volume>8</volume><issue>10</issue><spage>10328</spage><epage>10342</epage><pages>10328-10342</pages><issn>1936-0851</issn><eissn>1936-086X</eissn><abstract>Cerium oxide nanoparticles (nanoceria) are widely used in a variety of industrial applications including UV filters and catalysts. The expanding commercial scale production and use of ceria nanoparticles have inevitably increased the risk of release of nanoceria into the environment as well as the risk of human exposure. The use of nanoceria in biomedical applications is also being currently investigated because of its recently characterized antioxidative properties. In this study, we investigated the impact of ceria nanoparticles on the lysosome-autophagy system, the main catabolic pathway that is activated in mammalian cells upon internalization of exogenous material. We tested a battery of ceria nanoparticles functionalized with different types of biocompatible coatings (N-acetylglucosamine, polyethylene glycol and polyvinylpyrrolidone) expected to have minimal effect on lysosomal integrity and function. We found that ceria nanoparticles promote activation of the transcription factor EB, a master regulator of lysosomal function and autophagy, and induce upregulation of genes of the lysosome-autophagy system. We further show that the array of differently functionalized ceria nanoparticles tested in this study enhance autophagic clearance of proteolipid aggregates that accumulate as a result of inefficient function of the lysosome-autophagy system. This study provides a mechanistic understanding of the interaction of ceria nanoparticles with the lysosome-autophagy system and demonstrates that ceria nanoparticles are activators of autophagy and promote clearance of autophagic cargo. These results provide insights for the use of nanoceria in biomedical applications, including drug delivery. These findings will also inform the design of engineered nanoparticles with safe and precisely controlled impact on the environment and the design of nanotherapeutics for the treatment of diseases with defective autophagic function and accumulation of lysosomal storage material.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>25315655</pmid><doi>10.1021/nn505073u</doi><tpages>15</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1936-0851
ispartof ACS nano, 2014-10, Vol.8 (10), p.10328-10342
issn 1936-0851
1936-086X
language eng
recordid cdi_proquest_miscellaneous_1762057293
source American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
subjects Animals
Autophagy
Biomedical materials
Cell Line
Cerium - chemistry
Cerium oxide
Clearances
Coatings
Diseases
Humans
Lysosomes - chemistry
Metal Nanoparticles - chemistry
Microscopy, Electron, Transmission
Nanoparticles
Nanostructure
Risk
Surface Properties
title Ceria Nanoparticles Stabilized by Organic Surface Coatings Activate the Lysosome-Autophagy System and Enhance Autophagic Clearance
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T20%3A50%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Ceria%20Nanoparticles%20Stabilized%20by%20Organic%20Surface%20Coatings%20Activate%20the%20Lysosome-Autophagy%20System%20and%20Enhance%20Autophagic%20Clearance&rft.jtitle=ACS%20nano&rft.au=Song,%20Wensi&rft.date=2014-10-28&rft.volume=8&rft.issue=10&rft.spage=10328&rft.epage=10342&rft.pages=10328-10342&rft.issn=1936-0851&rft.eissn=1936-086X&rft_id=info:doi/10.1021/nn505073u&rft_dat=%3Cproquest_cross%3E1762057293%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-a414t-ac707fc5f309024c3815fd6f2a99b41947a3534ef07bec472c15c22df110d0fa3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1618140507&rft_id=info:pmid/25315655&rfr_iscdi=true