Single Protein Molecule Detection by Glass Nanopores

Nanopores can be used to detect and analyze single molecules in solution. We have used glass nanopores made by laser-assisted capillary-pulling, as a high-throughput and low cost method, to detect a range of label-free proteins: lysozyme, avidin, IgG, β-lactoglobulin, ovalbumin, bovine serum albumin...

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Bibliographic Details
Published in:ACS nano 2013-05, Vol.7 (5), p.4129-4134
Main Authors: Li, Wenhong, Bell, Nicholas A. W, Hernández-Ainsa, Silvia, Thacker, Vivek V, Thackray, Alana M, Bujdoso, Raymond, Keyser, Ulrich F
Format: Article
Language:English
Subjects:
Animals
Cattle
Glass
Glass - chemistry
Humans
Low cost
Lysozyme
Mammals
Molecular Weight
Nanopores
Nanostructure
Porosity
Prions
Protein Conformation
Proteins
Proteins - analysis
Proteins - chemistry
Signal-To-Noise Ratio
Solid state
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Summary:Nanopores can be used to detect and analyze single molecules in solution. We have used glass nanopores made by laser-assisted capillary-pulling, as a high-throughput and low cost method, to detect a range of label-free proteins: lysozyme, avidin, IgG, β-lactoglobulin, ovalbumin, bovine serum albumin (BSA), and β-galactosidase in solution. Furthermore, we show for the first time solid state nanopore measurements of mammalian prion protein, which in its abnormal form is associated with transmissible spongiform encephalopathies. Our approach provides a basis for protein characterization and the study of protein conformational diseases by nanopore detection.
ISSN:1936-0851
1936-086X
DOI:10.1021/nn4004567