Mithocondrial DNA clonality assay and P53 mutations in oral squamous cell carcinoma and matched neck metastases

The purpose of the present study was to see whether mtDNA D-loop analysis can be also applied in patients showing neck nodal metastasis to evaluate the presence or not of a clonal relationship with the index tumor. The study population consisted of 8 patients treated for a primary OSCC and presentin...

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Bibliographic Details
Published in:Annali di stomatologia 2014-04, Vol.5 (2), p.25-25
Main Authors: Gabusi, A, Gissi, D B, Sozzi, L, Foschini, M P, Morandi, L
Format: Article
Language:English
Subjects:
Cervical cancer
Deoxyribonucleic acid
DNA
Genes
Metastasis
Mitochondrial DNA
Mutations
Necks
Patients
Platforms
Synchronous
Tumors
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Summary:The purpose of the present study was to see whether mtDNA D-loop analysis can be also applied in patients showing neck nodal metastasis to evaluate the presence or not of a clonal relationship with the index tumor. The study population consisted of 8 patients treated for a primary OSCC and presenting synchronous neck-nodal metastases. Analysis of p53 mutations was additionally performed in each case to compare data obtained by the two different molecular techniques. DNA from exons 5-8 of the p53 gene was analyzed by deep DNA sequencing using 454 GS Junior platform. The results from mtDNA D-loop analysis revealed that 7 out of 8 cases (90%) of neck metastases were phylogenetically related with primary OSCC, while in the remaining one patient was not. The results from p53 analysis confirmed the absence of any relationship between neck-metastasis and OSCC in this patient and confirmed the clonal relationship between neck-metastasis and OSCC in other 3 patients, but was not informative in the remaining 4 patients.
ISSN:1824-0852
1971-1441