DNA interaction with cis- and trans- isomers of photosensitive surfactant

Interaction between DNA and photosensitive cationic surfactant in a solution is studied. Studies were conducted to examine the impact of the surfactant in its cis-conformation on the size of DNA molecule and also to investigate the phase behavior of the system depending on DNA and surfactant concent...

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Bibliographic Details
Published in:Journal of physics. Conference series 2014-01, Vol.572 (1), p.1-6
Main Authors: Unksov, I N, Kasyanenko, N A
Format: Article
Language:English
Subjects:
Cationic
Deoxyribonucleic acid
Isomers
Phase diagrams
Photosensitivity
Physics
Precipitation
Spectrophotometry
Surfactants
Ultraviolet radiation
Viscometry
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Summary:Interaction between DNA and photosensitive cationic surfactant in a solution is studied. Studies were conducted to examine the impact of the surfactant in its cis-conformation on the size of DNA molecule and also to investigate the phase behavior of the system depending on DNA and surfactant concentration. We conclude that trans- isomer of surfactant requires its smaller concentration to reach the DNA compaction compared with cis-isomer received by UV radiation of solutions. Studies of DNA-surfactant systems were performed by means of spectrophotometry and viscometry. Variation of surfactant concentration enables us to determine the precipitation zone on phase diagram. From the viscosity study it can be indicated that precipitation zone is narrower for UV-radiated surfactant and it shifts to higher surfactant concentration. Also we examine the reversibility of DNA compaction in systems with the surfactant in its trans- form.
ISSN:1742-6588
1742-6596
DOI:10.1088/1742-6596/572/1/012014