McCLEC, a robust and stable enzymatic based microreactor platform

A microfluidic chip for cross-linked enzyme crystals (McCLEC) is presented and demonstrated to be a stable, reusable and robust biocatalyst-based device with very promising biotechnological applications. The cost-effective microfluidic platform allows in situ crystallization, cross-linking and enzym...

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Bibliographic Details
Published in:Lab on a chip 2015-01, Vol.15 (20), p.4083-4089
Main Authors: Conejero-Muriel, Mayte, Rodríguez-Ruiz, Isaac, Martínez-Rodríguez, Sergio, Llobera, Andreu, Gavira, José A
Format: Article
Language:English
Subjects:
Amidohydrolases - chemistry
Amidohydrolases - metabolism
Animals
Bacillus cereus - enzymology
Biocatalysis
Chips
Crosslinking
Crystals
Devices
Enzyme Assays - instrumentation
Enzyme Stability
Lab-On-A-Chip Devices
Microfluidics
Muramidase - chemistry
Muramidase - metabolism
Platforms
Reuse
Storage stability
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Summary:A microfluidic chip for cross-linked enzyme crystals (McCLEC) is presented and demonstrated to be a stable, reusable and robust biocatalyst-based device with very promising biotechnological applications. The cost-effective microfluidic platform allows in situ crystallization, cross-linking and enzymatic reaction assays on a single device. A large number of enzymatic reuses of the McCLEC platform were achieved and a comparative analysis is shown illustrating the efficiency of the process and its storage stability for more than one year.
ISSN:1473-0197
1473-0189
DOI:10.1039/c5lc00776c