Synthesis of diacylglycerol analogs bearing photoaffinity tags for labelling mammalian diacylglycerol kinase

Signaling lipids such as diacylglycerol (DAG), phosphatidic acid, and the phosphatidylinositol polyphosphates are site-specific ligands for protein binding partners. Herein, we report the apotheoses of our initial approach to the development of diverse probes which are vital for understanding lipid-...

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Bibliographic Details
Published in:RSC advances 2015-01, Vol.5 (32), p.25457-25461
Main Authors: Eni, Sammy Eni, Rowland, Meng, Best, Michael D
Format: Article
Language:English
Subjects:
Binding
Kinases
Labelling
Ligands
Lipids
Mammals
Polyphosphates
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Summary:Signaling lipids such as diacylglycerol (DAG), phosphatidic acid, and the phosphatidylinositol polyphosphates are site-specific ligands for protein binding partners. Herein, we report the apotheoses of our initial approach to the development of diverse probes which are vital for understanding lipid-protein interactions. When incorporated into liposomes, these probes reduce mammalian diacylglycerol kinase's (DGK) enzymatic activity in a concentration, time, and light dependent manner.
ISSN:2046-2069
DOI:10.1039/c4ra16730a