Accumulation of transcription factors and cell signaling-related proteins in the nucleus during citrus–Xanthomonas interaction

The nucleus is the maestro of the cell and is involved in the modulation of cell signaling during stress. We performed a comprehensive nuclear proteome analysis of Citrus sinensis during interaction with host (Xanthomonas citri pv. citri-Xcc) and non-host (Xanthomonas oryzae pv. oryzae-Xoo) pathogen...

Full description

Saved in:
Bibliographic Details
Published in:Journal of plant physiology 2015-07, Vol.184, p.20-27
Main Authors: Rani, T. Swaroopa, Durgeshwar, P., Podile, Appa Rao
Format: Article
Language:English
Subjects:
2DE
Cell Nucleus - genetics
Cell Nucleus - metabolism
Chromatography, Liquid
Citrus
Citrus sinensis
Citrus sinensis - genetics
Citrus sinensis - immunology
Citrus sinensis - metabolism
Citrus sinensis - microbiology
Cyclopentanes - metabolism
Enrichment
Histones
Kinases
Nano-LC MS/MS
Nanostructure
Nuclei
Oxylipins - metabolism
Pathogens
Physiology
Plant Immunity
Plant Leaves - metabolism
Plant Proteins - genetics
Plant Proteins - metabolism
Proteins
Proteome - genetics
Proteome - metabolism
Proteome analysis
Species Specificity
Tandem Mass Spectrometry
Transcription Factors - genetics
Transcription Factors - metabolism
Xanthomonas
Xanthomonas - physiology
Xanthomonas oryzae
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The nucleus is the maestro of the cell and is involved in the modulation of cell signaling during stress. We performed a comprehensive nuclear proteome analysis of Citrus sinensis during interaction with host (Xanthomonas citri pv. citri-Xcc) and non-host (Xanthomonas oryzae pv. oryzae-Xoo) pathogens. The nuclear proteome was obtained using a sequential method of organelle enrichment and determined by nano-LC–MS/MS analysis. A total of 243 proteins accumulated differentially during citrus–Xanthomonas interaction, belonging to 11 functional groups, with signaling and transcription-related proteins dominating. MADS-box transcription factors, DEAD-box RNA helicase and leucine aminopeptidase, mainly involved in jasmonic acid (JA) responses, were in high abundance during non-host interaction (Xoo). Signaling-related proteins like serine/threonine kinase, histones (H3.2, H2A), phosphoglycerate kinase, dynamin, actin and aldolase showed increased accumulation early during Xoo interaction. Our results suggest that there is a possible involvement of JA-triggered defense responses during non-host resistance, with early recognition of the non-host pathogen.
ISSN:0176-1617
1618-1328
DOI:10.1016/j.jplph.2015.05.013