A highly sensitive electrochemical genosensor based on Co-porphyrin-labelled DNA

We report the use of Co-porphyrins as electrochemical tags for a highly sensitive and selective genosensor. An avian influenza virus-based DNA sequence characteristic of H5N1 was detected at femtomolar levels from competing non-complementary sequences through hybridisation with the labeled DNA.

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2014-04, Vol.50 (32), p.4196-4199
Main Authors: Grabowska, Iwona, Singleton, Daniel G, Stachyra, Anna, Góra-Sochacka, Anna, Sirko, Agnieszka, Zagórski-Ostoja, Włodzimierz, Radecka, Hanna, Stulz, Eugen, Radecki, Jerzy
Format: Article
Language:English
Subjects:
Animals
Biosensing Techniques
Deoxyribonucleic acid
DNA Probes - chemistry
DNA Probes - genetics
DNA, Viral - analysis
DNA, Viral - genetics
Electrochemistry - instrumentation
Electrochemistry - methods
Electrodes
Gene sequencing
Gold - chemistry
Influenza
Influenza A Virus, H5N1 Subtype - genetics
Nucleic Acid Hybridization
Porphyrins - chemistry
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Summary:We report the use of Co-porphyrins as electrochemical tags for a highly sensitive and selective genosensor. An avian influenza virus-based DNA sequence characteristic of H5N1 was detected at femtomolar levels from competing non-complementary sequences through hybridisation with the labeled DNA.
ISSN:1359-7345
1364-548X
DOI:10.1039/c4cc00172a