Prolactin gene polymorphism in Nili-Ravi buffaloes in relation to Sahiwal and Achai Cattle

In this study, prolactin gene polymorphism was investigated in Nili‐ Ravi buffaloes, Sahiwal and Achai cattle breeds, 100 per group, using polymerase chain reaction–restriction fragment length polymorphism (PCR‐RFLP) technique. Only genotype GG was observed in the case of Nili‐Ravi buffaloes. In Sah...

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Bibliographic Details
Published in:International journal of dairy technology 2013-02, Vol.66 (1), p.20-24
Main Authors: ISHAQ, RAFAQAT, SULEMAN, MUHAMMAD, RIAZ, MUHAMMAD NAEEM, YOUSAF, MUHAMMAD, SHAH, ABDULLAH, GHAFOOR, ABDUL
Format: Article
Language:English
Subjects:
Achai
Buffalo
Buffaloes
Cattle
Chain reactions
Chains (polymeric)
Dairy industry
Fragmentation
Genes
Genomics
International
Nili-Ravi
PCR-RFLP
Polymerase
Polymorphism
Sahiwal
Studies
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Summary:In this study, prolactin gene polymorphism was investigated in Nili‐ Ravi buffaloes, Sahiwal and Achai cattle breeds, 100 per group, using polymerase chain reaction–restriction fragment length polymorphism (PCR‐RFLP) technique. Only genotype GG was observed in the case of Nili‐Ravi buffaloes. In Sahiwal and Achai cattle, three genotypes were found, AA, AG and GG: the frequency of these genotypes were 72%, 18% and 10% in Sahiwal cattle and 44%, 34% and 22% in Achai cattle, respectively. The frequency of genotype AA was found to be higher in both cattle breeds. Results of chi‐square test at P 
ISSN:1364-727X
1471-0307
DOI:10.1111/j.1471-0307.2012.00875.x