The magnitude of spatial and temporal variation in delta 15N and delta 13C differs between taxonomic groups: Implications for food web studies

• Published in: Estuarine, coastal and shelf science coastal and shelf science, 2013-03, Vol.119, p.176-187
• Main Authors: Hyndes, Glenn A, Hanson, Christine E, Vanderklift, Mathew A
• Format: Article
• Language: English
• Subjects: Autotrophs; Brackish; Consistency; Consumers; Foods; Invertebrates; Isotopes; Marine; Shelves; Temporal logic
• ISSN: 0272-7714
• DOI: 10.1016/j.ecss.2012.12.015

Understanding variability in stable isotope abundance is essential for effective hypothesis testing and evaluating food sources, trophic levels and food web structure. The magnitude and sources of variability are likely to differ among taxonomic and functional groups. We aimed to quantify variability of delta 13C and delta 15N for 16 species representing seven distinct taxonomic groups of benthic invertebrates and autotrophs in a marine ecosystem. We quantified the magnitude of variability among individuals or shoots separated by metres, among eight sites separated by kilometres, and between two survey occasions separated by months. delta 13C varied by as much as 7ppt for primary producers, 4ppt for consumers, while delta 15N varied by as much as 9ppt and 2ppt respectively. Variation in delta 15N of seagrass was largely accounted for by differences among sites, while variation in delta 13C was mainly attributable to shoots collected a few metres apart. Compared to seagrasses, variation in macroalgae was mainly explained by differences between the two survey occasions for delta 15N and among individuals collected a few metres apart for delta 13C. Variation was generally lower for consumers and typically explained by differences among individuals for delta 15N but displayed inconsistent patterns for delta 13C. Dual isotope Bayesian mixing models showed that the potential contributions of food sources for herbivorous consumers varied among sites and between survey occasions, and also that there was high variability or uncertainty in the contributions of sources within sites. The relative consistency in the main sources of variation among broad taxonomic groups in autotrophs suggests that aspects of physiology that are phylogenetically conserved might be important influences on variation in natural abundances of stable isotopes. In comparison, the sources of variability were less consistent within and among broad consumer groups, suggesting complex interactions between consumers and their food sources.