Osteo mineralization of fibrin-decorated graphene oxide

Surface functionalized graphene oxide (GO) was synthesized by coating fibrin on its surface. The fibrin coated graphene oxide (FGO) was characterized for its physiochemical properties and its potential as an osteoinductive material was evaluated using osteoblast like cell line MG-63 and normal cell...

Full description

Saved in:
Bibliographic Details
Published in:Carbon (New York) 2013-05, Vol.56, p.64-76
Main Authors: Deepachitra, R., Chamundeeswari, M., Santhosh kumar, B., Krithiga, G., Prabu, P., Pandima Devi, M., Sastry, Thotapalli P.
Format: Article
Language:English
Subjects:
Biocompatibility
Biomedical materials
Biotechnology
Chemistry
Coating
Colloidal state and disperse state
Cross-disciplinary physics: materials science
rheology
Exact sciences and technology
Fibrin
General and physical chemistry
Graphene
Materials science
Nanocrystalline materials
Nanoscale materials and structures: fabrication and characterization
Oxides
Physical and chemical studies. Granulometry. Electrokinetic phenomena
Physics
Scanning electron microscopy
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Surface functionalized graphene oxide (GO) was synthesized by coating fibrin on its surface. The fibrin coated graphene oxide (FGO) was characterized for its physiochemical properties and its potential as an osteoinductive material was evaluated using osteoblast like cell line MG-63 and normal cell line NIH 3T3. The scanning electron microscopy (SEM) has confirmed the coating of fibrin on GO and the transmission electron microscopy (TEM) revealed both spherical and cubical nature of GO nanoparticles and FGO. In in vitro studies, FGO exhibited higher levels of alkaline phosphatase and calcium ion release which confirmed the osteoinductive nature of FGO. The 3-(4,5-dimethylthiazol-2-Y)-2,5-diphenyltetrazolium bromide (MTT) assay proved FGO as a biocompatible material. The results have suggested that FGO might be a promising scaffold for bone tissue engineering applications.
ISSN:0008-6223
1873-3891
DOI:10.1016/j.carbon.2012.12.070