In vitro evaluation of spray-dried chitosan microspheres crosslinked with pyromellitic dianhydride for oral colon-specific delivery of protein drugs

• Published in: Journal of applied polymer science 2014-08, Vol.131 (15), p.np-n/a
• Main Authors: Kavianinia, Iman, Plieger, Paul G., Kandile, Nadia G., Harding, David R. K.
• Format: Article
• Language: English
• Subjects: Applied sciences; Biological and medical sciences; Chitosan; Crosslinking; Dianhydrides; Drug delivery systems; Drugs; Exact sciences and technology; Forms of application and semi-finished materials; General pharmacology; Materials science; Medical sciences; Microspheres; Miscellaneous; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Polymer industry, paints, wood; Polymers; polysaccharides; Proteins; Serum albumin; swelling; Technology of polymers
• ISSN: 0021-8995; 1097-4628
• DOI: 10.1002/app.40514

ABSTRACT Chitosan microspheres have been prepared using a spray‐drying method, and crosslinked with pyromellitic dianhydride. The chemical structure of the modified chitosan was characterized by FTIR spectroscopy and solid state 13C NMR analysis. The particle size and morphology of the crosslinked chitosan were investigated. These microspheres were evaluated for colon‐specific delivery of bovine serum albumin (BSA) as a model protein drug. The results indicate that the drug was released as follows: 37.1 ± 2.8% after 2 h in SGF, 73.1 ± 4.8% after 8 h (2 h in SGF+ 6 h in SIF), and 80.9 ± 4.1% after 12 h in SCF. The effect of β‐glucosidase on the drug release was also examined. The encapsulation efficiency was decreased from 88.4 ± 3.1% to 62.8 ± 2.9%, with increasing BSA concentration. Loading capacity was significantly increased from 6.3 ± 0.3% to 41.8 ± 4.1% by increasing the initial BSA concentration. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40514.