The relationship between light intensity and nutrient uptake kinetics in six freshwater diatoms

• Published in: Journal of environmental sciences (China) 2015-08, Vol.34 (8), p.28-36
• Main Authors: Shi, Pengling, Shen, Hong, Wang, Wenjing, Chen, Wenjie, Xie, Ping
• Format: Article
• Language: English
• Subjects: Bacillariophyceae; China; Diatom; Diatoms - growth & development; Diatoms - metabolism; Diatoms - radiation effects; Eutrophication; Kinetics; Light; Light intensity; Michaelis–Menten equation; Phosphorus; Phosphorus - metabolism; Silicon; Silicon - metabolism; Species Specificity; Uptake kinetics; 光合活性; 光照强度; 养分; 半饱和常数; 吸收动力学; 底栖硅藻; 淡水; 细胞体积
• ISSN: 1001-0742; 1878-7320
• DOI: 10.1016/j.jes.2015.03.003

In order to find effective measures to control diatom blooms, a better understanding of the physiological characteristics of nutrient uptake in diatoms is needed. A study of P and Si-uptake kinetics for diatom species from two light regimes was conducted at low（LL）,moderate（ML） and high light intensities（HL）（2, 25 and 80 μmol photons/（m2·sec））,respectively. The results showed that P uptake of diatoms was heavily influenced by historic light regimes. P affinity changed with growth and photosynthetic activity. The lowest half saturation constant for P uptake（Km（P）） was under HL for high-light adapted diatoms while the lowest half-saturation constant for low-light adapted diatoms was observed under LL. The Si half-saturation constant（Km（Si）） increased with increasing light intensities for pennate diatoms but decreased for centric diatoms. Diatom volumes were correlated with the maximum Si uptake rates（Vm（Si）） at HL and K m（Si）at ML and HL for six diatom species. Our results imply that when we assess the development of diatom blooms we should consider light intensity and cell volume in addition to ambient Si or P concentration. The relationship between light intensity and P-uptake suggests that we can find suitable methods to control diatom blooms on the basis of reducing phytoplankton activity of P-uptake and photosynthesis simultaneously.