Metallothionein and Hsp70 trade-off against one another in Daphnia magna cross-tolerance to cadmium and heat stress

• Published in: Aquatic toxicology 2016-01, Vol.170, p.112-119
• Main Authors: Haap, Timo, Schwarz, Simon, Köhler, Heinz-R.
• Format: Article
• Language: English
• Subjects: Acclimation; Animals; Cadmium - toxicity; Daphnia - drug effects; Daphnia - metabolism; Daphnia clones; Daphnia magna; Drug Tolerance; Freshwater; Heat stress; Heavy metals; HSP70 Heat-Shock Proteins - metabolism; Metallothionein - genetics; Metallothionein - metabolism; Microevolution; Real-Time Polymerase Chain Reaction; Reproduction - drug effects; Temperature; Toxicity Tests, Acute; Water Pollutants, Chemical - toxicity
• ISSN: 0166-445X; 1879-1514
• DOI: 10.1016/j.aquatox.2015.11.008

•Cadmium acclimation of two Daphnia magna clones which differed in Cd sensitivity and Hsp70 levels.•Two distinct metal-handling strategies regarding Hsp70 and MT expression were observed.•High Hsp70 levels did not confer an increase in Cd and heat stress tolerance.•Our results indicate a trade-off between Hsp70 and MT. The association between the insensitivity of adapted ecotypes of invertebrates to environmental stress, such as heavy metal pollution, and overall low Hsp levels characterizing these organisms has been attracting attention in various studies. The present study seeks to induce and examine this phenomenon in Daphnia magna by multigenerational acclimation to cadmium in a controlled laboratory setting. In this experiment, interclonal variation was examined: two clones of D. magna that have previously been characterized to diverge regarding their cadmium resistance and levels of the stress protein Hsp70, were continuously exposed to a sublethal concentration of Cd over four generations to study the effects of acclimation on Hsp70, metallothionein (MT), reproduction and cross-tolerance to heat stress. The two clones differed in all the measured parameters in a characteristic way, clone T displaying Cd and heat resistance, lower Hsp70 levels and offspring numbers on the one hand and higher MT expression on the other hand, clone S the opposite for all these parameters. We observed only slight acclimation-induced changes in constitutive Hsp70 levels and reproductive output. The differences in MT expression between clones as well as between acclimated organisms and controls give evidence for MT accounting for the higher Cd tolerance of clone T. Overall high Hsp70 levels of clone S did not confer cross tolerance to heat stress, contrary to common expectations. Our results suggest a trade-off between the efforts to limit the proteotoxic symptoms of Cd toxicity by Hsp70 induction and those to sequester and detoxify Cd by means of MT.