The interaction of MMP-2/B7-H3 in human osteoporosis

Abstract The immune costimulatory molecule B7-H3 has been shown to be involved in the regulation of murine bone formation. However, the role of B7-H3 in bone metabolic diseases remains unknown. In our study, matrix metalloproteinase 2 (MMP-2) and soluble B7-H3 (sB7-H3) were found to be correlatively...

Full description

Saved in:
Bibliographic Details
Published in:Clinical immunology (Orlando, Fla.) Fla.), 2016-01, Vol.162, p.118-124
Main Authors: Feng, Ping, Zhang, Hong, Zhang, Zhuqiu, Dai, Xiaoli, Mao, Ting, Fan, Yinyin, Xie, Xiaofang, Wen, Huiyan, Yu, Peijuan, Hu, Yae, Yan, Ruhong
Format: Article
Language:English
Subjects:
Allergy and Immunology
B7 Antigens - antagonists & inhibitors
B7 Antigens - metabolism
Blotting, Western
Bone
Cell Line
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Fluorescent Antibody Technique
Humans
Matrix Metalloproteinase 2 - metabolism
Matrix metalloproteinases
Osteoblasts - metabolism
Osteoporosis
Osteoporosis - enzymology
Osteoporosis - physiopathology
Real-Time Polymerase Chain Reaction
RNA Interference
Soluble B7-H3
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract The immune costimulatory molecule B7-H3 has been shown to be involved in the regulation of murine bone formation. However, the role of B7-H3 in bone metabolic diseases remains unknown. In our study, matrix metalloproteinase 2 (MMP-2) and soluble B7-H3 (sB7-H3) were found to be correlatively up-regulated in the sera of osteoporosis patients. Furthermore, our results showed that MG63 cells treated with MMP-2 inhibitors produced lower amounts of sB7-H3 while cells with recombinant MMP-2 had an increased membrane B7-H3 (mB7-H3) shedding. Therefore, elevated MMP-2 levels resulted in an elevation of serum sB7-H3 and reduction of osteoblastic mB7-H3. B7-H3 knockdown in MG63 cells significantly decreased osteoblastic markers and substantially decreased the number of mineralized nodules after 21 days. Thus, B7-H3-deficient MG63 cells exhibited impaired bone formation. These results suggest that mB7-H3 is required for the later phases of osteoblast differentiation and that MMP-2/B7-H3 plays a negative regulatory role in osteoporosis.
ISSN:1521-6616
1521-7035
DOI:10.1016/j.clim.2015.11.009