Greater neurobehavioral deficits occur in adult mice after repeated, as compared to single, mild traumatic brain injury (mTBI)

• Published in: Behavioural brain research 2016-02, Vol.298 (Pt B), p.111-124
• Main Authors: Nichols, Jessica N., Deshane, Alok S., Niedzielko, Tracy L., Smith, Cory D., Floyd, Candace L.
• Format: Article
• Language: English
• Subjects: Animals; Anxiety Disorders - etiology; Anxiety Disorders - physiopathology; Behavior; Brain Injuries - complications; Brain Injuries - physiopathology; Cohort Studies; Depressive Disorder - etiology; Depressive Disorder - physiopathology; Disease Models, Animal; Exploratory Behavior; Learning and memory; Learning Disorders - etiology; Learning Disorders - physiopathology; Male; Maze Learning; Mice, Inbred C57BL; Mild; Motor Activity; Random Allocation; Repetitive; Sleep; Traumatic brain injury
• ISSN: 0166-4328; 1872-7549
• DOI: 10.1016/j.bbr.2015.10.052

•Mouse model of mTBI exhibited clinically relevant behavioral alterations.•Repeated mTBIs resulted in learning impairments.•Performance on species-typical behaviors worsened after repeated mTBIs.•Increased sleep was experienced after single and repeated mTBI.•Anxiety and depression were not observed after single or repeated mTBI. Mild traumatic brain injury (mTBI) accounts for the majority of all brain injuries and affected individuals typically experience some extent of cognitive and/or neuropsychiatric deficits. Given that repeated mTBIs often result in worsened prognosis, the cumulative effect of repeated mTBIs is an area of clinical concern and on-going pre-clinical research. Animal models are critical in elucidating the underlying mechanisms of single and repeated mTBI-associated deficits, but the neurobehavioral sequelae produced by these models have not been well characterized. Thus, we sought to evaluate the behavioral changes incurred after single and repeated mTBIs in mice utilizing a modified impact-acceleration model. Mice in the mTBI group received 1 impact while the repeated mTBI group received 3 impacts with an inter-injury interval of 24h. Classic behavior evaluations included the Morris water maze (MWM) to assess learning and memory, elevated plus maze (EPM) for anxiety, and forced swim test (FST) for depression/helplessness. Additionally, species-typical behaviors were evaluated with the marble-burying and nestlet shredding tests to determine motivation and apathy. Non-invasive vibration platforms were used to examine sleep patterns post-mTBI. We found that the repeated mTBI mice demonstrated deficits in MWM testing and poorer performance on species-typical behaviors. While neither single nor repeated mTBI affected behavior in the EPM or FST, sleep disturbances were observed after both single and repeated mTBI. Here, we conclude that behavioral alterations shown after repeated mTBI resemble several of the deficits or disturbances reported by patients, thus demonstrating the relevance of this murine model to study repeated mTBIs.