Molecular detection of Trypanosoma sp. and Blastocrithidia sp. (Trypanosomatidae) in phlebotomine sand flies (Psychodidae) in the Federal District of Brazil

INTRODUCTION : This study describes the occurrence of trypanosomatids in phlebotomines in Brasília, Brazil. METHODS : Two hundred and ten females of 13 sand fly species were analyzed by polymerase chain reaction (PCR) using different molecular markers (D7 24Sα rRNA, kDNA, and ITS1) and sequencing. R...

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Published in:Revista da Sociedade Brasileira de Medicina Tropical 2015-11, Vol.48 (6), p.776-779
Main Authors: Tauana, despaceSousa Ferreira, Thaís, Tâmara Castro Minuzzi-Souza, Andrey, José despace Andrade, Thais, Oliveira Coelho, Douglas, despaceAlmeida Rocha, Marcos, Takashi Obara, Hecht, Mariana, Nitz, Nadjar, Gurgel-Gonçalves, Rodrigo
Format: Article
Language:English
Subjects:
Blastocrithidia
Insects
Leishmania
Molecular biology
Polymerase chain reaction
Psychodidae
Studies
Trypanosoma
Trypanosomatidae
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Summary:INTRODUCTION : This study describes the occurrence of trypanosomatids in phlebotomines in Brasília, Brazil. METHODS : Two hundred and ten females of 13 sand fly species were analyzed by polymerase chain reaction (PCR) using different molecular markers (D7 24Sα rRNA, kDNA, and ITS1) and sequencing. RESULTS : PCR revealed trypanosomatid-positive samples from Nyssomyia whitmani and Evandromyia evandroi, which were negative by kDNA and ITS1 Leishmania-specific PCRs. DNA sequence analysis of D7 24Sα rRNA amplicons indicated the occurrence of Blastocrithidia sp. and Trypanosoma sp. in Nyssomyia whitmani and Evandromyia evandroi, respectively. CONCLUSIONS : Two trypanosomatid species other than Leishmania sp. were found to circulate in sand flies in Central Brazil.
ISSN:0037-8682
1678-9849
DOI:10.1590/0037-8682-0076-201